| Literature DB >> 7766013 |
T Petri1, G Langer, P Bringmann, L Cashion, S Shallow, W D Schleuning, P Donner.
Abstract
Salivary plasminogen activator from the vampire bat Desmodus rotundus (DSPA alpha 1) is a promising new thrombolytic agent. Continuous growth of a stably transfected, methotrexate amplified, dhfr- CHO cell line yields up to 60 mg l-1 of DSPA alpha 1. Utilizing an engineered baculovirus 10 mg l-1 were produced in batches of Sf9 insect cells. Recombinant DSPA alpha 1 is purified from both sources using a one-step purification protocol. Although differences in glycosylation were detected, enzymatic activity and fibrin cofactor dependency are unaffected when DSPA alpha 1 derived from the two expression systems is compared.Entities:
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Year: 1995 PMID： 7766013 DOI： 10.1016/0168-1656(94)00146-4
Source DB: PubMed Journal: J Biotechnol ISSN： 0168-1656 Impact factor: 3.307