Literature DB >> 7765935

Glycosylation and functional activity of anti-D secreted by two human lymphoblastoid cell lines.

D Cant1, J Barford, C Harbour, A Fletcher, N Packer, A Gooley.   

Abstract

Cell lines BTSN4 and BTSN5 were produced by the Epstein-Barr Virus (EBV) transformation of B-lymphocytes from the same human donor. Both secrete an anti-D monoclonal of the IgG1 subclass but these antibodies display vastly different effector activities. Specifically, anti-D from BTSN4 has a far greater activity in both monocyte- and lymphocyte-mediated ADCC reactions and causes a higher percentage of rosettes to be formed with monocyte-like U937 cells. This variation in functional activity is shown to coincide with changes in the structure of the sugar chains attached to the asparagine-297 site on the immunoglobulin heavy chain.

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Year:  1994        PMID： 7765935     DOI： 10.1007/BF00762397

Source DB:  PubMed          Journal:  Cytotechnology        ISSN： 0920-9069            Impact factor:   2.058


  15 in total

Review 1.  Environmental effects on protein glycosylation.

Authors:  C F Goochee; T Monica
Journal:  Biotechnology (N Y)       Date:  1990-05

2.  Functional activity of human Rh monoclonal antibodies (MABS).

Authors:  B M Kumpel
Journal:  J Immunogenet       Date:  1990 Aug-Oct

3.  Molecular definition of interaction sites on human IgG for Fc receptors (huFc gamma R).

Authors:  R Jefferis; J Lund; J Pound
Journal:  Mol Immunol       Date:  1990-12       Impact factor: 4.407

4.  Heterogeneity of IgG1 monoclonal anti-Rh(D): an investigation using ADCC and macrophage binding assays.

Authors:  S S Armstrong; E Wiener; S F Garner; S J Urbaniak; M Contreras
Journal:  Br J Haematol       Date:  1987-06       Impact factor: 6.998

5.  Synergistic effect of blending IgG1 and IgG3 monoclonal anti-D in promoting the metabolic response of monocytes to sensitized red cells.

Authors:  A G Hadley; B M Kumpel
Journal:  Immunology       Date:  1989-08       Impact factor: 7.397

6.  Ability of monoclonal anti-D antibodies to promote the binding of red cells to lymphocytes, granulocytes and monocytes.

Authors:  A H Merry; E Brojer; B Zupanska; A G Hadley; B M Kumpel; N C Hughes-Jones
Journal:  Vox Sang       Date:  1989       Impact factor: 2.144

7.  An in-vitro assessment of the functional activity of monoclonal anti-D.

Authors:  A G Hadley; B M Kumpel; K Leader; A H Merry; E Brojer; B Zupanska
Journal:  Clin Lab Haematol       Date:  1989

8.  Effector functions of a monoclonal aglycosylated mouse IgG2a: binding and activation of complement component C1 and interaction with human monocyte Fc receptor.

Authors:  R J Leatherbarrow; T W Rademacher; R A Dwek; J M Woof; A Clark; D R Burton; N Richardson; A Feinstein
Journal:  Mol Immunol       Date:  1985-04       Impact factor: 4.407

9.  Stable thiobarbituric acid chromophore with dimethyl sulphoxide. Application to sialic acid assay in analytical de-O-acetylation.

Authors:  L Skoza; S Mohos
Journal:  Biochem J       Date:  1976-12-01       Impact factor: 3.857

10.  Heterogeneity in the ability of IgG1 monoclonal anti-D to promote lymphocyte-mediated red cell lysis.

Authors:  B M Kumpel; K A Leader; A H Merry; A G Hadley; G D Poole; A Blancher; D Goossens; N C Hughes-Jones; B A Bradley
Journal:  Eur J Immunol       Date:  1989-12       Impact factor: 5.532
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