Low temperature cultivation--a step towards process optimisation.

Adherent recombinant BHK cells were cultivated at temperatures between 30 and 37 degrees C. Batch and repeated-batch-cultivations in a 2-litre bioreactor showed a significant influence on metabolism and cell growth. The low-temperature-cultivations showed a lower growth rate and a lower glucose consumption rate and, therefore, less lactate production. On the other hand, the maximum cell density and productivity seemed not to be affected by the temperature reduction.