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Literature DB >> 7765922

Selecting and designing cell lines for improved physiological characteristics.

J R Birch¹, R C Boraston, H Metcalfe, M E Brown, C R Bebbington, R P Field.

Abstract

We have developed several approaches to create cell lines with improved characteristics in cell culture. In some cases it has been possible to isolate natural variants with useful properties. Cholesterol independent variants of the mouse NSO myeloma cell line were isolated by cloning in a selective medium. A glutamine independent variant of a hyridoma was isolated by continuous (chemostat) culture under glutamine limited conditions in the presence of glutamate. Choline independent cells were isolated from a choline limited chemostat. In an alternative approach to modifying cell behaviour, we have used recombinant DNA techniques to introduce the glutamine synthetase (GS) gene to a hybridoma. This resulted in glutamine independence and increased productivity.

Entities: Chemical Disease Gene Species

Mesh：

Substances：

Year: 1994 PMID： 7765922 DOI： 10.1007/BF00762375

Source DB: PubMed Journal: Cytotechnology ISSN： 0920-9069 Impact factor: 2.058

11 in total

1. Enhancing monoclonal antibodies and hybridoma cell lines.

Authors: S S Seaver
Journal: Cytotechnology Date: 1992 Impact factor: 2.058

2. The uptake of amino acids by mouse cells (strain LS) during growth in batch culture and chemostat culture: the influence of cell growth rate.

Authors: J P Griffiths; S J Pirt
Journal: Proc R Soc Lond B Biol Sci Date: 1967-11-14

Review 3. Metabolic engineering--methodologies and future prospects.

Authors: G Stephanopoulos; A J Sinskey
Journal: Trends Biotechnol Date: 1993-09 Impact factor: 19.536

Review 4. Selection in chemostats.

Authors: D E Dykhuizen; D L Hartl
Journal: Microbiol Rev Date: 1983-06

5. Reduction of waste product excretion via nutrient control: Possible strategies for maximizing product and cell yields on serum in cultures of mammalian cells.

Authors: M W Glacken; R J Fleischaker; A J Sinskey
Journal: Biotechnol Bioeng Date: 1986-09 Impact factor: 4.530

6. Cholesterol requirement for growth of IR983F and P3X63-Ag8-U1 myeloma cells in serum-free medium.

Authors: J L Li; Y J Li; Y J Chao; L X Lin; M H Ouyang; Y B Peng; W S Chang
Journal: Cytobios Date: 1991

7. Development of a serum-free medium for growth of NS-1 mouse myeloma cells and its application to the isolation of NS-1 hybridomas.

Authors: T Kawamoto; J D Sato; A Le; D B McClure; G H Sato
Journal: Anal Biochem Date: 1983-04-15 Impact factor: 3.365

8. High-level expression of a recombinant antibody from myeloma cells using a glutamine synthetase gene as an amplifiable selectable marker.

Authors: C R Bebbington; G Renner; S Thomson; D King; D Abrams; G T Yarranton
Journal: Biotechnology (N Y) Date: 1992-02

9. Effects of proximate cholesterol precursors and steroid hormones on mouse myeloma growth in serum-free medium.

Authors: J D Sato; H T Cao; Y Kayada; M C Cabot; G H Sato; T Okamoto; C J Welsh
Journal: In Vitro Cell Dev Biol Date: 1988-12

10. The effects of adapting human diploid cells to grow in glutamic acid media on cell morphology, growth and metabolism.

Authors: J B Griffiths
Journal: J Cell Sci Date: 1973-03 Impact factor: 5.285

5 in total

Selecting and designing cell lines for improved physiological characteristics.

1. Enhancing monoclonal antibodies and hybridoma cell lines.

2. The uptake of amino acids by mouse cells (strain LS) during growth in batch culture and chemostat culture: the influence of cell growth rate.

Review 3. Metabolic engineering--methodologies and future prospects.

Review 4. Selection in chemostats.

5. Reduction of waste product excretion via nutrient control: Possible strategies for maximizing product and cell yields on serum in cultures of mammalian cells.

6. Cholesterol requirement for growth of IR983F and P3X63-Ag8-U1 myeloma cells in serum-free medium.

7. Development of a serum-free medium for growth of NS-1 mouse myeloma cells and its application to the isolation of NS-1 hybridomas.

8. High-level expression of a recombinant antibody from myeloma cells using a glutamine synthetase gene as an amplifiable selectable marker.

9. Effects of proximate cholesterol precursors and steroid hormones on mouse myeloma growth in serum-free medium.

10. The effects of adapting human diploid cells to grow in glutamic acid media on cell morphology, growth and metabolism.

1. Medium design for insect cell culture.

2. Proteomic profiling of recombinant cells from large-scale mammalian cell culture processes.

3. Modification of glucose and glutamine metabolism in hybridoma cells through metabolic engineering.

4. Development of Animal-free, Protein-Free and Chemically-Defined Media for NS0 Cell Culture.

5. Advances in animal cell recombinant protein production: GS-NS0 expression system.