The effect of cell-culture conditions on the oligosaccharide structures of secreted glycoproteins.

Glycoprotein oligosaccharide structure influences numerous important protein properties. In recent years, a number of studies have demonstrated that cell-culture methodology can significantly affect the oligosaccharide structures of recombinant proteins and antibodies, and, in the past year in particular, several of the specific environmental variables responsible for these effects have been identified.