Literature DB >> 7765449

Inhibition of DNAse activity in PFGE analysis of DNA from Campylobacter jejuni.

J R Gibson1, K Sutherland, R J Owen.   

Abstract

DNAse-positive strains of Campylobacter jejuni degrade their chromosomal DNA during standard preparative procedures before pulsed-field gel electrophoresis (PFGE). A simple method for inactivation of this DNAse activity is described. Formaldehyde fixation of the bacterial cells resulted in the preservation of the DNA in a state suitable for restriction digestion and subsequent electrophoretic analysis.

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Year:  1994        PMID： 7765449     DOI： 10.1111/j.1472-765x.1994.tb00474.x

Source DB:  PubMed          Journal:  Lett Appl Microbiol        ISSN： 0266-8254            Impact factor:   2.858


  25 in total

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3.  Comparative fingerprinting analysis of Campylobacter jejuni subsp. jejuni strains by amplified-fragment length polymorphism genotyping.

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4.  Stability of related human and chicken Campylobacter jejuni genotypes after passage through chick intestine studied by pulsed-field gel electrophoresis.

Authors:  M L Hänninen; M Hakkinen; H Rautelin
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5.  Prevalence of Helicobacter pullorum in conventional, organic, and free-range broilers and typing of isolates.

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7.  Molecular subtyping scheme for Salmonella panama.

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Review 8.  Inhibition and facilitation of nucleic acid amplification.

Authors:  I G Wilson
Journal:  Appl Environ Microbiol       Date:  1997-10       Impact factor: 4.792

9.  Molecular subtyping scheme for serotypes HS1 and HS4 of Campylobacter jejuni.

Authors:  R J Owen; K Sutherland; C Fitzgerald; J Gibson; P Borman; J Stanley
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10.  Typing of human Campylobacter jejuni isolates in Finland by pulsed-field gel electrophoresis.

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Journal:  J Clin Microbiol       Date:  1998-06       Impact factor: 5.948
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