Literature DB >> 7765242

Media replenishment: a tool for the analysis of high-cell density perfusion systems.

M D Mahadevan1, J A Klimkowsky, Y M Deo.   

Abstract

A simple method to analyze media consumption and by-product build-up in high-cell-density perfusion cultures has been developed. This technique makes use of a media replenishment strategy which can be used to decouple the two phenomena. Also, the replenished media method can be used to identify limitation by complex and undefined media components such as bovine serum.

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Year:  1994        PMID: 7765242     DOI: 10.1007/BF00758173

Source DB:  PubMed          Journal:  Cytotechnology        ISSN: 0920-9069            Impact factor:   2.058


  5 in total

1.  Manipulation of heterogeneous hybridoma cultures for overproduction of monoclonal antibodies.

Authors:  K L McKinney; R Dilwith; G Belfort
Journal:  Biotechnol Prog       Date:  1991 Sep-Oct

2.  Effects of ammonia and lactate on hybridoma growth, metabolism, and antibody production.

Authors:  S S Ozturk; M R Riley; B O Palsson
Journal:  Biotechnol Bioeng       Date:  1992-02-20       Impact factor: 4.530

3.  Transient kinetics of hybridoma growth and monoclonal antibody production in serum-limited cultures.

Authors:  M Dalili; D F Ollis
Journal:  Biotechnol Bioeng       Date:  1989-03       Impact factor: 4.530

4.  A kinetic analysis of hybridoma growth and metabolism in batch and continuous suspension culture: effect of nutrient concentration, dilution rate, and pH.

Authors:  W M Miller; H W Blanch; C R Wilke
Journal:  Biotechnol Bioeng       Date:  1988-10-05       Impact factor: 4.530

5.  Growth kinetics of hybridoma cells: (2) The effects of varying energy source concentrations.

Authors:  K Low; C Harbour
Journal:  Dev Biol Stand       Date:  1985
  5 in total
  2 in total

1.  Continuous, high capacity reconstitution of nutrient media from concentrated intermediates.

Authors:  D W Jayme; J M Kubiak; T A Battistoni; D J Cady
Journal:  Cytotechnology       Date:  1996-01       Impact factor: 2.058

2.  Basal medium development for serum-free culture: a historical perspective.

Authors:  D Jayme; T Watanabe; T Shimada
Journal:  Cytotechnology       Date:  1997-01       Impact factor: 2.058

  2 in total

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