Literature DB >> 7765239

A simple HPLC technique for accurate monitoring of mammalian cell metabolism.

T Stoll1, P Pugeaud, U von Stockar, I W Marison.   

Abstract

We have developed a simple and accurate isocratic HPLC method, without any prederivatisation, for the determination of glucose, lactate, glutamine, glutamate, pyrrolidone carboxylic acid and alanine in samples from mammalian cell cultures. The method has been successfully validated with enzyme analysis for each of the compounds. Quantification of pyrrolidone carboxylic acid makes the correction for glutamine decrease due to chemical decomposition very simple and accurate, and avoids some possibly erroneous calculations.

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Year:  1994        PMID： 7765239     DOI： 10.1007/BF00758177

Source DB:  PubMed          Journal:  Cytotechnology        ISSN： 0920-9069            Impact factor:   2.058


  6 in total

1.  Chemical decomposition of glutamine in cell culture media: effect of media type, pH, and serum concentration.

Authors:  S S Ozturk; B O Palsson
Journal:  Biotechnol Prog       Date:  1990 Mar-Apr

2.  Spontaneous decomposition of glutamine in cell culture media.

Authors:  G L TRITSCH; G E MOORE
Journal:  Exp Cell Res       Date:  1962-11       Impact factor: 3.905

3.  Effect of anions on the non-enzymatic desamidation of glutamine.

Authors:  J B GILBERT; V E PRICE; J P GREENSTEIN
Journal:  J Biol Chem       Date:  1949-08       Impact factor: 5.157

4.  Asparaginase and glutaminase activities in culture media containing dialyzed fetal calf serum.

Authors:  J Wein; I E Goetz
Journal:  In Vitro       Date:  1973 Nov-Dec

5.  Growth inhibition in animal cell culture. The effect of lactate and ammonia.

Authors:  T Hassell; S Gleave; M Butler
Journal:  Appl Biochem Biotechnol       Date:  1991-07       Impact factor: 2.926

6.  High-performance liquid chromatographic determination of metabolic products for fermentation control of mammalian cell culture: analysis of carbohydrates, organic acids and orthophosphate using refractive index and ultraviolet detectors.

Authors:  F Weigang; M Reiter; A Jungbauer; H Katinger
Journal:  J Chromatogr       Date:  1989-12-29
  6 in total
  2 in total

1.  Monitoring of Microphysiological Systems: Integrating Sensors and Real-Time Data Analysis toward Autonomous Decision-Making.

Authors:  Ashlyn T Young; Kristina R Rivera; Patrick D Erb; Michael A Daniele
Journal:  ACS Sens       Date:  2019-04-19       Impact factor: 7.711

2.  Production of immunoglobulin A in different reactor configurations.

Authors:  T Stoll; C Perregaux; U von Stockar; I W Marison
Journal:  Cytotechnology       Date:  1995-02       Impact factor: 2.058
  2 in total

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