Literature DB >> 7765117

A rapid method for measuring organelle-specific substrate transport in homogenates from plant tissues.

U I Flügge1, A Weber.   

Abstract

A rapid method is described for measuring organelle-specific metabolite transport systems in crude homogenates from plants. The tissues were homogenized in liquid nitrogen, extracted with buffer and reconstituted into artificial membranes. The method allowed demonstration of the known different substrate specificities of chloroplast triose phosphate/phosphate translocators from C3- and C4-plants, of the triose phosphate/phosphate translocator from non-green tissue, and of the dicarboxylate translocator. It thus by-passes the necessity to isolate intact plant organelles and, in addition, only a low amount of tissue material is required for transport measurements.

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Year:  1994        PMID： 7765117

Source DB:  PubMed          Journal:  Planta        ISSN： 0032-0935            Impact factor:   4.116


  13 in total

Review 1.  Molecular aspects of isolated and reconstituted carrier proteins from animal mitochondria.

Authors:  R Krämer; F Palmieri
Journal:  Biochim Biophys Acta       Date:  1989-04-17

2.  COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.

Authors:  D I Arnon
Journal:  Plant Physiol       Date:  1949-01       Impact factor: 8.340

3.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

4.  Comparison of the kinetic properties, inhibition and labelling of the phosphate translocators from maize and spinach mesophyll chloroplasts.

Authors:  A Gross; G Brückner; H W Heldt; U I Flügge
Journal:  Planta       Date:  1990-01       Impact factor: 4.116

5.  Reconstitution of adenine nucleotide transport with purified ADP, ATP-carrier protein.

Authors:  R Krämer; M Klingenberg
Journal:  FEBS Lett       Date:  1977-10-15       Impact factor: 4.124

6.  A Two-Translocator Model for the Transport of 2-Oxoglutarate and Glutamate in Chloroplasts during Ammonia Assimilation in the Light.

Authors:  K C Woo; U I Flügge; H W Heldt
Journal:  Plant Physiol       Date:  1987-07       Impact factor: 8.340

7.  Purification and functional reconstitution of the 2-oxoglutarate/malate translocator from spinach chloroplasts.

Authors:  E Menzlaff; U I Flügge
Journal:  Biochim Biophys Acta       Date:  1993-04-08

8.  Antisense repression of the chloroplast triose phosphate translocator affects carbon partitioning in transgenic potato plants.

Authors:  J W Riesmeier; U I Flügge; B Schulz; D Heineke; H W Heldt; L Willmitzer; W B Frommer
Journal:  Proc Natl Acad Sci U S A       Date:  1993-07-01       Impact factor: 11.205

9.  Specific transport of inorganic phosphate, 3-phosphoglycerate and triosephosphates across the inner membrane of the envelope in spinach chloroplasts.

Authors:  R Fliege; U I Flügge; K Werdan; H W Heldt
Journal:  Biochim Biophys Acta       Date:  1978-05-10

10.  Cloning and in vivo expression of functional triose phosphate/phosphate translocators from C3- and C4-plants: evidence for the putative participation of specific amino acid residues in the recognition of phosphoenolpyruvate.

Authors:  K Fischer; B Arbinger; B Kammerer; C Busch; S Brink; H Wallmeier; N Sauer; C Eckerskorn; U I Flügge
Journal:  Plant J       Date:  1994-02       Impact factor: 6.417
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  10 in total

1.  Evidence for the expression of the triosephosphate translocator gene in green and non-green tissue of tomato and potato.

Authors:  D Schünemann; K Schott; S Borchert; H W Heldt
Journal:  Plant Mol Biol       Date:  1996-04       Impact factor: 4.076

2.  Molecular characterization of a carbon transporter in plastids from heterotrophic tissues: the glucose 6-phosphate/phosphate antiporter.

Authors:  B Kammerer; K Fischer; B Hilpert; S Schubert; M Gutensohn; A Weber; U I Flügge
Journal:  Plant Cell       Date:  1998-01       Impact factor: 11.277

3.  A new class of plastidic phosphate translocators: a putative link between primary and secondary metabolism by the phosphoenolpyruvate/phosphate antiporter.

Authors:  K Fischer; B Kammerer; M Gutensohn; B Arbinger; A Weber; R E Häusler; U I Flügge
Journal:  Plant Cell       Date:  1997-03       Impact factor: 11.277

4.  EST-analysis of the thermo-acidophilic red microalga Galdieria sulphuraria reveals potential for lipid A biosynthesis and unveils the pathway of carbon export from rhodoplasts.

Authors:  Andreas P M Weber; Christine Oesterhelt; Wolfgang Gross; Andrea Bräutigam; Lori A Imboden; Inga Krassovskaya; Nicole Linka; Julia Truchina; Jörg Schneidereit; Hildegard Voll; Lars M Voll; Marc Zimmermann; Aziz Jamai; Wayne R Riekhof; Bin Yu; R Michael Garavito; Christoph Benning
Journal:  Plant Mol Biol       Date:  2004-05       Impact factor: 4.076

5.  Arabidopsis SAMT1 defines a plastid transporter regulating plastid biogenesis and plant development.

Authors:  Florence Bouvier; Nicole Linka; Jean-Charles Isner; Jérôme Mutterer; Andreas P M Weber; Bilal Camara
Journal:  Plant Cell       Date:  2006-11-10       Impact factor: 11.277

6.  Peroxisomal ATP import is essential for seedling development in Arabidopsis thaliana.

Authors:  Nicole Linka; Frederica L Theodoulou; Richard P Haslam; Marc Linka; Jonathan A Napier; H Ekkehard Neuhaus; Andreas P M Weber
Journal:  Plant Cell       Date:  2008-12-10       Impact factor: 11.277

7.  Functional characterization of the plastidic phosphate translocator gene family from the thermo-acidophilic red alga Galdieria sulphuraria reveals specific adaptations of primary carbon partitioning in green plants and red algae.

Authors:  Marc Linka; Aziz Jamai; Andreas P M Weber
Journal:  Plant Physiol       Date:  2008-09-17       Impact factor: 8.340

8.  Analysis of carbohydrate transport across the envelope of isolated cauliflower-bud amyloplasts.

Authors:  T Möhlmann; O Batz; U Maass; H E Neuhaus
Journal:  Biochem J       Date:  1995-04-15       Impact factor: 3.857

9.  Defects in leaf carbohydrate metabolism compromise acclimation to high light and lead to a high chlorophyll fluorescence phenotype in Arabidopsis thaliana.

Authors:  Jessica Schmitz; Mark Aurel Schöttler; Stephan Krueger; Stefan Geimer; Anja Schneider; Tatjana Kleine; Dario Leister; Kirsten Bell; Ulf-Ingo Flügge; Rainer E Häusler
Journal:  BMC Plant Biol       Date:  2012-01-16       Impact factor: 4.215

10.  The Xylulose 5-Phosphate/Phosphate Translocator Supports Triose Phosphate, but Not Phosphoenolpyruvate Transport Across the Inner Envelope Membrane of Plastids in Arabidopsis thaliana Mutant Plants.

Authors:  Elke J A Hilgers; Pia Staehr; Ulf-Ingo Flügge; Rainer E Häusler
Journal:  Front Plant Sci       Date:  2018-10-18       Impact factor: 5.753
  10 in total

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