| Literature DB >> 7765052 |
N Ponelies1, J Scheef, A Harim, G Leitz, K O Greulich.
Abstract
The use of lasers for complete micromanipulation of metaphase chromosomes, cells and subcellular structures is reviewed. DNA probes from single microdissected chromosome segments can be prepared using Alu or Adaptor PCR. In plant biotechnology, laser microsurgery can be used to prepare non-enzymatically protoplasts from Medicago sativa. Microgravity can be simulated in the alga Chara by lifting intracellular gravity transmitting elements with the optical tweezers.Entities:
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Year: 1994 PMID： 7765052 DOI： 10.1016/0168-1656(94)90030-2
Source DB: PubMed Journal: J Biotechnol ISSN： 0168-1656 Impact factor: 3.307