| Literature DB >> 7764702 |
J Frère1.
Abstract
Rapid screening and large-scale plasmid DNA isolation procedures are described for lactic acid bacteria, using glass beads to break cells. The rapid screening procedure allows one to obtain plasmid DNA pellets in less than 1 h. This method has been successfully tested on various bacteria from the genera Lactococcus, Leuconostoc, Lactobacillus, Pediococcus, Streptococcus, Enterococcus and Propionibacterium. This procedure yields plasmid DNA with minor chromosomal and plasmid DNA-degraded form contaminations.Entities:
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Year: 1994 PMID： 7764702 DOI： 10.1111/j.1472-765x.1994.tb00853.x
Source DB: PubMed Journal: Lett Appl Microbiol ISSN： 0266-8254 Impact factor: 2.858