Literature DB >> 7764343

Expression of the cellulase (FI-CMCase) gene of Aspergillus aculeatus in Escherichia coli.

T Ooi1, K Minamiguchi, T Kawaguchi, H Okada, S Murao, M Arai.   

Abstract

FI-CMCase cDNA of Aspergillus aculeatus was expressed in Escherichia coli by using the tac promoter of E. coli. Transformants of E. coli harboring a plasmid pHEM06 containing mature form FI-CMCase cDNA produced FI-CMCase in the cytoplasm of the cells. The enzyme from E. coli cells was purified to yield 56% and it was immunological identical to that of FI-CMCase purified from A. aculeatus.

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Year:  1993        PMID： 7764343     DOI： 10.1271/bbb.57.1960

Source DB:  PubMed          Journal:  Biosci Biotechnol Biochem        ISSN： 0916-8451            Impact factor:   2.043


  2 in total

1.  Crystallization and preliminary X-ray diffraction analysis of an endo-1,4-β-D-glucanase from Aspergillus aculeatus F-50.

Authors:  Yun Chen; Jian Wen Huang; Chun Chi Chen; Hui Lin Lai; Jian Jin; Rey Ting Guo
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2015-03-20       Impact factor: 1.056

2.  Molecular characterization and heterologous expression of the gene encoding a low-molecular-mass endoglucanase from Trichoderma reesei QM9414.

Authors:  H Okada; K Tada; T Sekiya; K Yokoyama; A Takahashi; H Tohda; H Kumagai; Y Morikawa
Journal:  Appl Environ Microbiol       Date:  1998-02       Impact factor: 4.792
  2 in total

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