Literature DB >> 7764326

An isologous porcine promoter permits high level expression of human hemoglobin in transgenic swine.

A Sharma1, M J Martin, J F Okabe, R A Truglio, N K Dhanjal, J S Logan, R Kumar.   

Abstract

We describe isologous promoter replacement as an approach to permit high level expression of human hemoglobin in transgenic swine. We linked the human beta globin genomic coding region to the porcine beta globin promoter and used this fusion gene in an expression construct containing the human beta locus control region and the human alpha and epsilon genes to produce transgenic pigs. The highest level of expression was 24% human (32g/liter) and 30% human alpha/pig beta hybrid (40g/liter) hemoglobin in one transgenic pig. This pig was bred to a non-transgenic animal resulting in the transmission of high level human hemoglobin expression to 5 of 12 progeny.

Entities:  

Mesh：

Substances：

Year:  1994        PMID： 7764326     DOI： 10.1038/nbt0194-55

Source DB:  PubMed          Journal:  Biotechnology (N Y)        ISSN： 0733-222X


  15 in total

Review 1.  Biomedical and agricultural applications of animal transgenesis.

Authors:  Alison J Thomson; Jim McWhir
Journal:  Mol Biotechnol       Date:  2004-07       Impact factor: 2.695

Review 2.  The transgenic animal platform for biopharmaceutical production.

Authors:  L R Bertolini; H Meade; C R Lazzarotto; L T Martins; K C Tavares; M Bertolini; J D Murray
Journal:  Transgenic Res       Date:  2016-01-28       Impact factor: 2.788

3.  Circe's haemoglobins, pig-human hybrids: functional characterization and structural considerations.

Authors:  M T Sanna; B Giardina; M Pellegrini; A Olianas; I Messana; M Castagnola; M Corda
Journal:  Biochem J       Date:  1998-10-15       Impact factor: 3.857

4.  Chimeric hemoglobins--hybrids of human and swine hemoglobin: assembly and stability of interspecies hybrids.

Authors:  M J Rao; B N Manjula; R Kumar; A S Acharya
Journal:  Protein Sci       Date:  1996-05       Impact factor: 6.725

Review 5.  Genetic modifications of pigs for medicine and agriculture.

Authors:  Jeffrey J Whyte; Randall S Prather
Journal:  Mol Reprod Dev       Date:  2011-06-10       Impact factor: 2.609

Review 6.  Development of recombinant hemoglobin-based oxygen carriers.

Authors:  Cornelius L Varnado; Todd L Mollan; Ivan Birukou; Bryan J Z Smith; Douglas P Henderson; John S Olson
Journal:  Antioxid Redox Signal       Date:  2012-11-16       Impact factor: 8.401

Review 7.  Transgenesis in the rat and larger mammals.

Authors:  L J Mullins; J J Mullins
Journal:  J Clin Invest       Date:  1996-04-01       Impact factor: 14.808

8.  Porcine germline genome engineering.

Authors:  Luhan Yang; George Church; Hong-Ye Zhao; Lusheng Huang; Yangbin Gao; Hong-Jiang Wei; Geoffrey Yang
Journal:  Proc Natl Acad Sci U S A       Date:  2021-04-30       Impact factor: 11.205

9.  Phenotypic and genotypic stability of multiple lines of transgenic pigs expressing recombinant human protein C.

Authors:  K E Van Cott; H Lubon; C G Russell; S P Butler; F C Gwazdauskas; J Knight; W N Drohan; W H Velander
Journal:  Transgenic Res       Date:  1997-05       Impact factor: 2.788

10.  High-efficiency synthesis of human alpha-endorphin and magainin in the erythrocytes of transgenic mice: a production system for therapeutic peptides.

Authors:  A Sharma; A M Khoury-Christianson; S P White; N K Dhanjal; W Huang; C Paulhiac; E J Friedman; B N Manjula; R Kumar
Journal:  Proc Natl Acad Sci U S A       Date:  1994-09-27       Impact factor: 11.205
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.