| Literature DB >> 7764095 |
J D Marks1, W H Ouwehand, J M Bye, R Finnern, B D Gorick, D Voak, S J Thorpe, N C Hughes-Jones, G Winter.
Abstract
We have isolated human antibody fragments with binding specificities against the blood group antigens of the ABO and I blood group systems (B and HI), of the Rh system (D and E) and of the Kell system (Kpb), by selecting a phage-antibody library on human red cells. The fragments, expressed in bacteria, were antigen-specific and effective in assays including agglutination and immunohistochemistry. Selection of phage-antibody libraries using intact cells seems to offer a promising alternative to hybridoma technology for the production of antibodies against cell surface molecules.Entities:
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Year: 1993 PMID： 7764095 DOI： 10.1038/nbt1093-1145
Source DB: PubMed Journal: Biotechnology (N Y) ISSN： 0733-222X