Literature DB >> 7764022

Site-directed mutagenesis of chitinase from Alteromonas sp. strain O-7.

H Tsujibo1, H Orikoshi, C Imada, Y Okami, K Miyamoto, Y Inamori.   

Abstract

Chitinase (Chi85) from Alteromonas sp. strain O-7 contains the two conserved regions common to microbial and plant chitinases. We did site-directed mutagenesis of Chi85 to investigate the effects of the conserved amino acid residues on chitinase activity. We suggest that Asp-290 and Glu-292 of Chi85 may be the essential amino acid residues for the cleavage of beta-glycosidic linkage of chitin.

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Year:  1993        PMID： 7764022     DOI： 10.1271/bbb.57.1396

Source DB:  PubMed          Journal:  Biosci Biotechnol Biochem        ISSN： 0916-8451            Impact factor:   2.043


  6 in total

1.  Structural insights into the catalytic mechanism of a family 18 exo-chitinase.

Authors:  D M van Aalten; D Komander; B Synstad; S Gåseidnes; M G Peter; V G Eijsink
Journal:  Proc Natl Acad Sci U S A       Date:  2001-07-31       Impact factor: 11.205

2.  Srchi13, a novel early nodulin from Sesbania rostrata, is related to acidic class III chitinases.

Authors:  S Goormachtig; S Lievens; W Van de Velde; M Van Montagu; M Holsters
Journal:  Plant Cell       Date:  1998-06       Impact factor: 11.277

3.  Characterization of chitinase C from a marine bacterium, Alteromonas sp. strain O-7, and its corresponding gene and domain structure.

Authors:  H Tsujibo; H Orikoshi; K Shiotani; M Hayashi; J Umeda; K Miyamoto; C Imada; Y Okami; Y Inamori
Journal:  Appl Environ Microbiol       Date:  1998-02       Impact factor: 4.792

4.  Cloning, sequencing, and expression of the gene encoding Clostridium paraputrificum chitinase ChiB and analysis of the functions of novel cadherin-like domains and a chitin-binding domain.

Authors:  K Morimoto; S Karita; T Kimura; K Sakka; K Ohmiya
Journal:  J Bacteriol       Date:  1997-12       Impact factor: 3.490

5.  The broad bean gene VfNOD32 encodes a nodulin with sequence similarities to chitinases that is homologous to (alpha/beta)8-barrel-type seed proteins.

Authors:  A M Perlick; M Frühling; G Schröder; S C Frosch; A Pühler
Journal:  Plant Physiol       Date:  1996-01       Impact factor: 8.340

6.  Bioconversion of chitin and concomitant production of chitinase and N-acetylglucosamine by novel Achromobacter xylosoxidans isolated from shrimp waste disposal area.

Authors:  Kumaran Subramanian; Balamurugan Sadaiappan; Wilson Aruni; Alagappan Kumarappan; Rajasekar Thirunavukarasu; Guru Prasad Srinivasan; Selvaraj Bharathi; Prasannabalaji Nainangu; Pugazhvendan Sampath Renuga; Anandajothi Elamaran; Deivasigamani Balaraman; Mahendran Subramanian
Journal:  Sci Rep       Date:  2020-07-17       Impact factor: 4.379
  6 in total

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