Literature DB >> 7763862

Isozyme specific polymerase chain reaction analysis of differential gene expression: a general method applied to lignin peroxidase genes of Phanerochaete chrysosporium.

P Brooks1, P Sims, P Broda.   

Abstract

Analysis of differential expression of closely related genes is a general problem. The lignin peroxidase genes of Phanerochaete chrysosporium represent a typical case. They are differentially expressed according to the conditions encountered during growth. We show that the expression of two such genes, LIG1 and LIG5, can be differentiated at the mRNA level using a highly specific form of the polymerase chain reaction. This generally applicable method allows rapid and accurate analysis of complex and closely related mRNA populations.

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Year:  1993        PMID： 7763862     DOI： 10.1038/nbt0793-830

Source DB:  PubMed          Journal:  Biotechnology (N Y)        ISSN： 0733-222X


  9 in total

1.  Organization and differential regulation of a cluster of lignin peroxidase genes of Phanerochaete chrysosporium.

Authors:  P Stewart; D Cullen
Journal:  J Bacteriol       Date:  1999-06       Impact factor: 3.490

2.  Reverse transcription-PCR analysis of the regulation of the manganese peroxidase gene family.

Authors:  J M Gettemy; B Ma; M Alic; M H Gold
Journal:  Appl Environ Microbiol       Date:  1998-02       Impact factor: 4.792

3.  Substrate-dependent differential splicing of introns in the regions encoding the cellulose binding domains of two exocellobiohydrolase I-like genes in Phanerochaete chrysosporium.

Authors:  P R Birch; P F Sims; P Broda
Journal:  Appl Environ Microbiol       Date:  1995-10       Impact factor: 4.792

4.  Temporal expression of the major lignin peroxidase genes of Phanerochaete chrysosporium.

Authors:  K Boominathan; T M D'Souza; P S Naidu; C Dosoretz; C A Reddy
Journal:  Appl Environ Microbiol       Date:  1993-11       Impact factor: 4.792

5.  Identification of genes for lignin peroxidases and manganese peroxidases in ectomycorrhizal fungi.

Authors:  David M Chen; Andrew F S Taylor; Ron M Burke; John W G Cairney
Journal:  New Phytol       Date:  2001-10       Impact factor: 10.323

6.  Isolation, characterization, and analysis of the expression of the cbhII gene of Phanerochaete chrysosporium.

Authors:  C A Tempelaars; P R Birch; P F Sims; P Broda
Journal:  Appl Environ Microbiol       Date:  1994-12       Impact factor: 4.792

7.  Chromosome polymorphisms close to the cm-ADE1 locus of candida maltosa.

Authors:  D Becher; S Schulze; A Kasüske; R Stoll; H Wedler; S G Oliver
Journal:  Mol Gen Genet       Date:  1995-06-10

8.  PCR-mediated analysis of lignocellulolytic gene transcription by Phanerochaete chrysosporium: substrate-dependent differential expression within gene families.

Authors:  P Broda; P R Birch; P R Brooks; P F Sims
Journal:  Appl Environ Microbiol       Date:  1995-06       Impact factor: 4.792

9.  Hydrocarbon biodegradation and transcriptome responses of cellulase, peroxidase, and laccase encoding genes inhabiting rhizospheric fungal isolates.

Authors:  Mayasar I Al-Zaban; Maha A AlHarbi; Mohamed A Mahmoud
Journal:  Saudi J Biol Sci       Date:  2021-01-21       Impact factor: 4.219
  9 in total

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