Binding kinetics of antigen by immobilized antibody or of antibody by immobilized antigen: influence of lateral interactions and variable rate coefficients.

Kinetic rate expressions are developed for the binding kinetics of antigen by immobilized antibody or of antibody by immobilized antigen. The influence of lateral interactions leads to variable rate coefficients for adsorption, and these provide a more realistic picture of the events occurring on the surface. Emphasis is placed on the similarities and dissimilarities present in the kinetic rate expressions. A general trend in the rate expressions is observed on going from dual-step binding to dual-step binding with lateral interactions. This provides physical insights into the reactions occurring at the surface and helps in their control and stability at the surface.