| Literature DB >> 7763525 |
C von der Osten1, S Branner, S Hastrup, L Hedegaard, M D Rasmussen, H Bisgård-Frantzen, S Carlsen, J M Mikkelsen.
Abstract
Microbial proteases are used extensively in a large number of industrial processes and most importantly in detergent formulations facilitating the removal of proteinaceous stains. Site-directed mutagenesis has been employed in the construction of subtilisin variants with improved storage and oxidation stabilities. It is shown that in spite of significant structural homology between subtilisins subjected to protein engineering the effects of specific mutations can be quite different. Mutations that stabilize one subtilisin may destabilize another.Mesh：
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Year: 1993 PMID： 7763525 DOI： 10.1016/0168-1656(93)90125-7
Source DB: PubMed Journal: J Biotechnol ISSN： 0168-1656 Impact factor: 3.307