Literature DB >> 7762805

Quantitative polymerase chain reaction assay for aggrecan and link protein gene expression in cartilage.

P Re1, W B Valhmu, M Vostrejs, D S Howell, S G Fischer, A Ratcliffe.   

Abstract

A procedure has been developed to quantify the levels of aggrecan and link protein mRNAs in small amounts of various tissues, including cartilage, using the power of PCR to amplify extremely low levels of specific templates. The PCR protocol which was selected allows for a simple assay procedure, with standards in different tubes from the samples. This straightforward procedure is quantitative, inexpensive, and allows for many samples to be analyzed at one time.

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Year:  1995        PMID： 7762805     DOI： 10.1006/abio.1995.1169

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  4 in total

1.  Transforming growth factor beta one (TGF-beta 1) enhancement of the chondrocytic phenotype in aged perichondrial cells: an in vitro study.

Authors:  M C Lee; R S Goomer; K Takahashi; F L Harwood; M Amiel; D Amiel
Journal:  Iowa Orthop J       Date:  2000

2.  myo-Inositol 1,4,5-trisphosphate and Ca(2+)/calmodulin-dependent factors mediate transduction of compression-induced signals in bovine articular chondrocytes.

Authors:  Wilmot B Valhmu; Frank J Raia
Journal:  Biochem J       Date:  2002-02-01       Impact factor: 3.857

3.  Quantification of aggrecan and link-protein mRNA in human articular cartilage of different ages by competitive reverse transcriptase-PCR.

Authors:  M C Bolton; J Dudhia; M T Bayliss
Journal:  Biochem J       Date:  1996-10-15       Impact factor: 3.857

4.  Methods of high integrity RNA extraction from cell/agarose construct.

Authors:  Takahiro Ogura; Akihiro Tsuchiya; Tom Minas; Shuichi Mizuno
Journal:  BMC Res Notes       Date:  2015-11-04
  4 in total

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