| Literature DB >> 7757243 |
D Lutomski1, R Joubert-Caron, P Bourin, D Bladier, M Caron.
Abstract
A method for the purification and biotinylation of Fab fragments, using thiophilic adsorption (T-gel), is described. The T-gel was used to purify an IgG fraction directly in the buffer suitable for biotinylation, and to adsorb intact IgGs and papain after enzymatic digestion. For the final step, Fc fragments were removed with a protein A column.Entities:
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Year: 1995 PMID： 7757243 DOI： 10.1016/0378-4347(94)00399-p
Source DB: PubMed Journal: J Chromatogr B Biomed Appl ISSN： 1572-6495