Literature DB >> 775637

Human pancreatic islets in culture: effects of supplementing the medium with homologous and heterologous serum.

H Goldman, E Colle.   

Abstract

Islets of Langerhans, isolated from the human fetal pancreas and cultured in media supplemented with serums from human umbilical cord or adult donors, remained intact, free-floating, and functional for 2 to 5 months. Substitution of fetal calf serum for the human serum usually resulted in attachment of islets, monolayer formation, and relatively early (3 weeks) functional arrest.

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Year:  1976        PMID： 775637     DOI： 10.1126/science.775637

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  15 in total

1.  Long-term effect of pH on B-cell function in isolated islets of Langerhans in tissue culture.

Authors:  J Brunstedt; J H Nielsen
Journal:  Diabetologia       Date:  1978-09       Impact factor: 10.122

2.  Isolated mouse pancreatic islets in culture: effects of serum and different culture media on the insulin production of the islets.

Authors:  A Andersson
Journal:  Diabetologia       Date:  1978-06       Impact factor: 10.122

3.  Development in vitro of epithelial-cell monolayers derived from fetal rat pancreas.

Authors:  D H Wallace; O D Hegre
Journal:  In Vitro       Date:  1979-04

4.  Cell culture and in vitro studies of fresh and cryopreserved human insulinoma.

Authors:  A W Saxe; J W Yoon; P Gorden; M F Brennan
Journal:  In Vitro       Date:  1982-10

Review 5.  Pancreas and islet transplantation. II. Clinical trials.

Authors:  D E Sutherland
Journal:  Diabetologia       Date:  1981-04       Impact factor: 10.122

Review 6.  Pancreas and islet transplantation.

Authors:  D E Sutherland; A J Matas; J S Najarian
Journal:  World J Surg       Date:  1977-03       Impact factor: 3.352

Review 7.  Pancreas and islet transplantation.

Authors:  J S Najarian; F C Goetz; D E Sutherland
Journal:  Jpn J Surg       Date:  1982

8.  Organ culture of the Islets of Langerhans from young and senescent rats.

Authors:  C Remacle; L De Clercq; P Delaère; M C Many; A Gommers
Journal:  Cell Tissue Res       Date:  1980       Impact factor: 5.249

9.  Possible toxic effects of normal and diabetic patient serum on pancreatic B-cells.

Authors:  A Lernmark; J Sehlin; I B Täljedal; H Kromann; J Nerup
Journal:  Diabetologia       Date:  1978-01-14       Impact factor: 10.122

10.  Preservation of beta cell function in adult human pancreatic islets for several months in vitro.

Authors:  J H Nielsen; J Brunstedt; A Andersson; C Frimodt-Møller
Journal:  Diabetologia       Date:  1979-02       Impact factor: 10.122
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