| Literature DB >> 7755967 |
J Paulsen1, D Ochs, M Harder, C Duvos, H Mayer, E Wingender.
Abstract
Human parathyroid hormone (hPTH) has been bacterially expressed in bioreactors as cro-beta-galactosidase-hPTH fusion protein. We have developed a large-scale purification scheme that exploits the pH-dependent differential solubility of hPTH and a two-step chromatographic procedure. We demonstrate that in a number of assay systems, the recombinant material obtained by this procedure is biologically active.Entities:
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Year: 1995 PMID： 7755967 DOI： 10.1016/0168-1656(95)00002-8
Source DB: PubMed Journal: J Biotechnol ISSN： 0168-1656 Impact factor: 3.307