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Literature DB >> 775315

Stabilization and size of araC protein.

Abstract

AraC protein from Escherichia coli has been further stabilized and characterized. pH is a critical variable in conferring stability. araC protein has a sedimentation coefficient of 4.0 +/- 0.2s on standardized 5%-20% glycerol gradients. Its isoelectric point is at a pH of 7.1.

Entities: Chemical Gene Species

Mesh：

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Year: 1976 PMID： 775315 DOI： 10.1007/bf00331563

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

7 in total

1. A method for determining the sedimentation behavior of enzymes: application to protein mixtures.

Authors: R G MARTIN; B N AMES
Journal: J Biol Chem Date: 1961-05 Impact factor: 5.157

2. Interaction of the regulatory gene product with the operator site in the L-arabinose operon of Escherichia coli.

Authors: G Wilcox; K J Clemetson; P Cleary; E Englesberg
Journal: J Mol Biol Date: 1974-01-05 Impact factor: 5.469

3. In vitro activation of the transcription of araBAD operon by araC activator.

Authors: N Lee; G Wilcox; W Gielow; J Arnold; P Cleary; E Englesberg
Journal: Proc Natl Acad Sci U S A Date: 1974-03 Impact factor: 11.205

4. Regulation of the L-arabinose operon BAD in vitro.

Authors: G Wilcox; P Meuris; R Bass; E Englesberg
Journal: J Biol Chem Date: 1974-05-10 Impact factor: 5.157

5. Synthesis of the arabinose operon regulator protein in a cell-free system.

Authors: H L Yang; G Zubay
Journal: Mol Gen Genet Date: 1973-04-12

Review 6. Regulation: positive control.

Authors: E Englesberg; G Wilcox
Journal: Annu Rev Genet Date: 1974 Impact factor: 16.830

7. Examination of the dissociation of multichain proteins in guanidine hydrochloride by membrane osmometry.

Authors: F J Castellino; R Barker
Journal: Biochemistry Date: 1968-06 Impact factor: 3.162

7 in total

8 in total

1. In vivo DNA-binding and oligomerization properties of the Shigella flexneri AraC-like transcriptional regulator VirF as identified by random and site-specific mutagenesis.

Authors: Megan E Porter; Charles J Dorman
Journal: J Bacteriol Date: 2002-01 Impact factor: 3.490

Review 2. Arac/XylS family of transcriptional regulators.

Authors: M T Gallegos; R Schleif; A Bairoch; K Hofmann; J L Ramos
Journal: Microbiol Mol Biol Rev Date: 1997-12 Impact factor: 11.056

3. Mutations in the araC regulatory gene of Escherichia coli B/r that affect repressor and activator functions of AraC protein.

Authors: L G Cass; G Wilcox
Journal: J Bacteriol Date: 1986-06 Impact factor: 3.490

Stabilization and size of araC protein.

1. A method for determining the sedimentation behavior of enzymes: application to protein mixtures.

2. Interaction of the regulatory gene product with the operator site in the L-arabinose operon of Escherichia coli.

3. In vitro activation of the transcription of araBAD operon by araC activator.

4. Regulation of the L-arabinose operon BAD in vitro.

5. Synthesis of the arabinose operon regulator protein in a cell-free system.

Review 6. Regulation: positive control.

7. Examination of the dissociation of multichain proteins in guanidine hydrochloride by membrane osmometry.

1. In vivo DNA-binding and oligomerization properties of the Shigella flexneri AraC-like transcriptional regulator VirF as identified by random and site-specific mutagenesis.

Review 2. Arac/XylS family of transcriptional regulators.

3. Mutations in the araC regulatory gene of Escherichia coli B/r that affect repressor and activator functions of AraC protein.

4. Regulatory properties of araC(c) mutants in the L-arabinose operon of escherichia coliB/r.

5. Autoregulation of the tyrR gene.

6. Functional domains of the AraC protein.

7. Variation of half-site organization and DNA looping by AraC protein.

Review 8. Positively regulated bacterial expression systems.