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Literature DB >> 775306

Identification of a mutation within the structural gene for the a subunit of DNA-dependent RNA polymerase of E. coli.

H Fujiki, P Palm, W Zillig, R Calendar, M Sunshine.

Abstract

An E. coli mutant rpoA109 unable to support the growth of phage P2 produces DNA-dependent RNA polymerase with an altered alpha subunit. Histidine is substituted for leucine in one tryptic peptide from the mutant alpha subunit. The existence of only one rpoA gene within the E. coli chromosome is indicated.

Entities: Chemical Species

Mesh：

Substances：

Year: 1976 PMID： 775306 DOI： 10.1007/bf00331552

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

13 in total

1. The subunits of DNA-dependent RNA polymerase from E. coli: I. Amino acid analysis and primary structure of the N-terminal regions.

Authors: H Fujiki; G Zurek
Journal: FEBS Lett Date: 1975-07-15 Impact factor: 4.124

2. Reconstitution of bacterial DNA-dependent RNA-polymerase from isolated subunits as a tool for the elucidation of the role of the subunits in transcription.

Authors: A Heil; W Zillig
Journal: FEBS Lett Date: 1970-12 Impact factor: 4.124

3. Disk electrophoresis of basic proteins and peptides on polyacrylamide gels.

Authors: R A REISFELD; U J LEWIS; D E WILLIAMS
Journal: Nature Date: 1962-07-21 Impact factor: 49.962

Review 4. Strategy and tactics in protein chemistry.

Authors: B S Hartley
Journal: Biochem J Date: 1970-10 Impact factor: 3.857

5. A new method of large scale preparation of highly purified DNA-dependent RNA-polymerase from E. coli.

Authors: W Zillig; K Zechel; H J Halbwachs
Journal: Hoppe Seylers Z Physiol Chem Date: 1970-02

6. Factor stimulating transcription by RNA polymerase.

Authors: R R Burgess; A A Travers; J J Dunn; E K Bautz
Journal: Nature Date: 1969-01-04 Impact factor: 49.962

7. Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.

Authors: P Cuatrecasas
Journal: J Biol Chem Date: 1970-06 Impact factor: 5.157

8. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

9. Identification of a gene for the alpha-subunit of RNA polymerase at the str-spc region of the Escherichia coli chromosome.

Authors: S R Jaskunas; R R Burgess; M Nomura
Journal: Proc Natl Acad Sci U S A Date: 1975-12 Impact factor: 11.205

10. Combinations of specific color reactions useful in the peptide mapping technique.

Authors: C W Easley
Journal: Biochim Biophys Acta Date: 1965-09-13

33 in total

1. Lambda transducing bacteriophage carrying deletions of the argCBH-rpoBC region of the Escherichia coli chromosome.

Authors: T Linn; M Goman; J Scaife
Journal: J Bacteriol Date: 1979-11 Impact factor: 3.490

2. Sequence analysis of two temperature-sensitive mutations in the alpha subunit gene (rpoA) of Escherichia coli RNA polymerase.

Authors: K Igarashi; N Fujita; A Ishihama
Journal: Nucleic Acids Res Date: 1990-10-25 Impact factor: 16.971

Identification of a mutation within the structural gene for the a subunit of DNA-dependent RNA polymerase of E. coli.

1. The subunits of DNA-dependent RNA polymerase from E. coli: I. Amino acid analysis and primary structure of the N-terminal regions.

2. Reconstitution of bacterial DNA-dependent RNA-polymerase from isolated subunits as a tool for the elucidation of the role of the subunits in transcription.

3. Disk electrophoresis of basic proteins and peptides on polyacrylamide gels.

Review 4. Strategy and tactics in protein chemistry.

5. A new method of large scale preparation of highly purified DNA-dependent RNA-polymerase from E. coli.

6. Factor stimulating transcription by RNA polymerase.

7. Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.

8. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

9. Identification of a gene for the alpha-subunit of RNA polymerase at the str-spc region of the Escherichia coli chromosome.

10. Combinations of specific color reactions useful in the peptide mapping technique.

1. Lambda transducing bacteriophage carrying deletions of the argCBH-rpoBC region of the Escherichia coli chromosome.

2. Sequence analysis of two temperature-sensitive mutations in the alpha subunit gene (rpoA) of Escherichia coli RNA polymerase.

3. Escherichia coli K-12 and B contain functional bacteriophage P2 ogr genes.

4. Gene for the alpha subunit of Bacillus subtilis RNA polymerase maps in the ribosomal protein gene cluster.

5. Control of bacteriophage P2 gene expression: analysis of transcription of the ogr gene.

6. Mutations in rpoA affect expression of anaerobically regulated genes in Salmonella typhimurium.

7. Mutational analysis of a bacteriophage P4 late promoter.

8. A temperature-sensitive mutant of Escherichia coli affected in the alpha subunit of RNA polymerase.

9. Chromosomal location of a structural gene for the RNA polymerase sigma factor in Escherichia coli.

10. A new host gene (groPC) necessary for lambda DNA replication.