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Literature DB >> 775132

Function of gene 49 of bacteriophage T4. I. Isolation and biochemical characterization of very fast-sedimenting DNA.

Abstract

Very fast-sedimenting DNA was isolated from cells after infection with gene 49 defective phage T4. This DNA appeared membrane bound throughout the time after infection and could be isolated either in the membrane-bound form (M-DNA) or free of membrane (released DNA) depending on the lysis procedure. Released DNA formed complexes of marked stability with sedimentation velocities between 1,400S and 2,100S. These complexes did not seem to contain material other than DNA. This was concluded from the results of RNA, protein, and membrane labeling experiments and density analysis. In addition, these complexes were resistant against treatment with n-butanol, phenol. chloroform-methanol, sodium dodecyl sulfate, Sarkosyl, Pronase, RNase, or lysozyme. The observation that more then 90% of the purified very fast-sedimenting DNA is retrapped by magnesium-Sarkosyl crystals (M-band) suggests that the M-band technique may not be sufficient as a test for DNA-membrane attachment.

Entities: Chemical Disease Species

Mesh：

Substances：
DNA, Viral

Year: 1976 PMID： 775132 PMCID： PMC354799

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

22 in total

1. Association of replicative T4 deoxyribonucleic acid and bacterial membranes.

Authors: R C Miller
Journal: J Virol Date: 1972-11 Impact factor: 5.103

2. On the structure of the folded chromosome of Escherichia coli.

Authors: A Worcel; E Burgi
Journal: J Mol Biol Date: 1972-11-14 Impact factor: 5.469

3. Effect of inhibition of macromolecule synthesis on the association of bacteriophage T4 DNA with membrane.

Authors: C F Earhart; C J Sauri; G Fletcher; J L Wulff
Journal: J Virol Date: 1973-04 Impact factor: 5.103

4. An electron microscopic analysis of pathways for bacteriophage T4 DNA recombination.

Authors: T R Broker
Journal: J Mol Biol Date: 1973-11-25 Impact factor: 5.469

Review 5. The role of the host cell membrane in the replication and morphogenesis of bacteriophages.

Authors: P J Siegel; M Schaechter
Journal: Annu Rev Microbiol Date: 1973 Impact factor: 15.500

6. Bacteriophage T4 head morphogenesis. II. Studies on the maturation of gene 49-defective head intermediates.

Authors: R B Luftig; C Ganz
Journal: J Virol Date: 1972-02 Impact factor: 5.103

7. Maturation of the head of bacteriophage T4. III. DNA packaging into preformed heads.

Authors: U K Laemmli; N Teaff; J D'Ambrosia
Journal: J Mol Biol Date: 1974-10-05 Impact factor: 5.469

8. Visualization of replicating mammalian and T4 bacteriophage DNA.

Authors: J A Huberman
Journal: Cold Spring Harb Symp Quant Biol Date: 1968

9. Studies on T4-induced nucleases. Isolation and characterization of a manganese-activated T4-induced endonuclease.

Authors: B Kemper; J Hurwitz
Journal: J Biol Chem Date: 1973-01-10 Impact factor: 5.157

10. Bacteriophage T4 head maturation: release of progeny DNA from the host cell membrane.

Authors: P J Siegel; M Schaechter
Journal: J Virol Date: 1973-03 Impact factor: 5.103

16 in total

1. Genetic recombination in bacteriophage T4: single-burst analysis of cosegregants and evidence in favor of a splice/patch coupling model.

Authors: V P Shcherbakov; L A Plugina; M A Nesheva
Journal: Genetics Date: 1992-08 Impact factor: 4.562

2. Bacteriophage T4D head morphogenesis. VIII. DNA-protein associations in intermediate head structures that accumulate in gene 49--mutant-infected cells.

Authors: S J Glinert; R B Luftig
Journal: J Virol Date: 1977-06 Impact factor: 5.103

Function of gene 49 of bacteriophage T4. I. Isolation and biochemical characterization of very fast-sedimenting DNA.

1. Association of replicative T4 deoxyribonucleic acid and bacterial membranes.

2. On the structure of the folded chromosome of Escherichia coli.

3. Effect of inhibition of macromolecule synthesis on the association of bacteriophage T4 DNA with membrane.

4. An electron microscopic analysis of pathways for bacteriophage T4 DNA recombination.

Review 5. The role of the host cell membrane in the replication and morphogenesis of bacteriophages.

6. Bacteriophage T4 head morphogenesis. II. Studies on the maturation of gene 49-defective head intermediates.

7. Maturation of the head of bacteriophage T4. III. DNA packaging into preformed heads.

8. Visualization of replicating mammalian and T4 bacteriophage DNA.

9. Studies on T4-induced nucleases. Isolation and characterization of a manganese-activated T4-induced endonuclease.

10. Bacteriophage T4 head maturation: release of progeny DNA from the host cell membrane.

1. Genetic recombination in bacteriophage T4: single-burst analysis of cosegregants and evidence in favor of a splice/patch coupling model.

2. Bacteriophage T4D head morphogenesis. VIII. DNA-protein associations in intermediate head structures that accumulate in gene 49--mutant-infected cells.

3. Resolution of single and double Holliday junction recombination intermediates by GEN1.

4. Partial purification of an enzyme from Saccharomyces cerevisiae that cleaves Holliday junctions.

5. Effects of DNA heterologies on bacteriophage lambda packaging.

6. Folded, concatenated genomes as replication intermediates of bacteriophage T7 DNA.

7. Structures of bulged three-way DNA junctions.

8. UV irradiation impairs in vivo encapsidation of bacteriophage T4 DNA.

9. Genetic control of formation of very fast sedimenting DNA of bacteriophage T4.

10. Modulation of the packaging reaction of bacteriophage t4 terminase by DNA structure.