Literature DB >> 7750146

In vitro transcription from the Nectria haematococca PDA1 promoter in an homologous extract reflects in vivo pisatin-responsive regulation.

Y Ruan1, D C Straney.   

Abstract

The PDA1 gene of Nectria haematococca MP VI (anamorph: Fusarium solani) encodes pisatin demethylase. This enzyme detoxifies the isoflavanoid phytoalexin pisatin produced by the plant on which this fungus is pathogenic. Expression of pisatin demethylase activity is induced in a mycelium by pretreatment with pisatin. We have developed homologous in vitro system which accurately initiates transcription from the PDA1 promoter. Transcription levels in vitro reflect the same pisatin-responsive stimulation as measured for PDA1 mRNA in vivo, and are dependent upon sequences in the 5' upstream region of PDA1. Pisatin-responsive transcription from the PDA1 promoter indicates that initiation of transcription is a major regulatory step in the pisatin induction of pisatin demethylase expression.

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Year:  1994        PMID： 7750146     DOI： 10.1007/BF00326578

Source DB:  PubMed          Journal:  Curr Genet        ISSN： 0172-8083            Impact factor:   3.886


  18 in total

1.  A potent GAL4 derivative activates transcription at a distance in vitro.

Authors:  M Carey; J Leatherwood; M Ptashne
Journal:  Science       Date:  1990-02-09       Impact factor: 47.728

2.  Factors involved in specific transcription by human RNA polymerase II: analysis by a rapid and quantitative in vitro assay.

Authors:  M Sawadogo; R G Roeder
Journal:  Proc Natl Acad Sci U S A       Date:  1985-07       Impact factor: 11.205

Review 3.  The control of transcription in Saccharomyces cerevisiae.

Authors:  C Stanway; A J Kingsman; S M Kingsman
Journal:  Bioessays       Date:  1987-08       Impact factor: 4.345

4.  PCR-based construction of promoter/G-free templates for in vitro transcription analysis allows selection of plasmids with optimal activity in homologous extracts.

Authors:  Y Ruan; D C Straney
Journal:  Gene       Date:  1994-09-02       Impact factor: 3.688

5.  Inhibition of in vivo and in vitro transcription by monoclonal antibodies prepared against wheat germ RNA polymerase II that react with the heptapeptide repeat of eukaryotic RNA polymerase II.

Authors:  N E Thompson; T H Steinberg; D B Aronson; R R Burgess
Journal:  J Biol Chem       Date:  1989-07-05       Impact factor: 5.157

6.  Accurate initiation at RNA polymerase II promoters in extracts from Saccharomyces cerevisiae.

Authors:  N F Lue; R D Kornberg
Journal:  Proc Natl Acad Sci U S A       Date:  1987-12       Impact factor: 11.205

7.  Solubilization and Reconstitution of Pisatin Demethylase, a Cytochrome P-450 from the Pathogenic Fungus Nectria haematococca.

Authors:  A E Desjardins; D E Matthews; H D Vanetten
Journal:  Plant Physiol       Date:  1984-07       Impact factor: 8.340

8.  A gene from the fungal plant pathogen Nectria haematococca that encodes the phytoalexin-detoxifying enzyme pisatin demethylase defines a new cytochrome P450 family.

Authors:  A P Maloney; H D VanEtten
Journal:  Mol Gen Genet       Date:  1994-06-03

9.  Activation of yeast RNA polymerase II transcription by a thymidine-rich upstream element in vitro.

Authors:  N F Lue; A R Buchman; R D Kornberg
Journal:  Proc Natl Acad Sci U S A       Date:  1989-01       Impact factor: 11.205

10.  Binding site-dependent direct activation and repression of in vitro transcription by Drosophila homeodomain proteins.

Authors:  Y Ohkuma; M Horikoshi; R G Roeder; C Desplan
Journal:  Cell       Date:  1990-05-04       Impact factor: 41.582
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  1 in total

1.  Identification of elements in the PDA1 promoter of Nectria haematococca necessary for a high level of transcription in vitro.

Authors:  Y Ruan; D C Straney
Journal:  Mol Gen Genet       Date:  1996-01-15
  1 in total

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