| Literature DB >> 7745247 |
A Carrier1, F Ducancel, N B Settiawan, L Cattolico, B Maillère, M Léonetti, P Drevet, A Ménez, J C Boulain.
Abstract
We have designed an expression vector permitting the production in the periplasm of Escherichia coli of a fusion protein comprising a dimer of bacterial alkaline phosphatase (PhoA) and two Fab or scFv fragments of a monoclonal antibody directed against human IgG. Each hybrid protein expressed both high specificity for the antigen and full PhoA activity. We show that crude periplasmic extracts containing these conjugates can be used as such in enzyme immunoassays for the detection of human IgG, as exemplified in the case of anti-hepatitis B immunoglobulin.Entities:
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Year: 1995 PMID： 7745247 DOI： 10.1016/0022-1759(94)00344-v
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303