| Literature DB >> 7738906 |
S Saito1, T Kasahara, S Sakakura, H Umekage, N Harada, M Ichijo.
Abstract
It has been reported that interleukin-8 (IL-8) is secreted from the placental and decidual tissues and that IL-8 levels in the amniotic fluids are significantly elevated by chorioamnionitis or labor pain. The present study was aimed at defining the localization of IL-8 mRNA as well as IL-8 protein at the feto-maternal interface using in situ hybridization and immunohistochemical staining. Both IL-8 mRNA and protein were localized in cytotrophoblast, syncytiotrophoblast and Hofbauer cells of the placenta, decidual stromal cells, decidual lymphocytes and endometrial gland cells. IL-8 secretion from glandular cells has not previously been reported. In addition, we confirmed IL-8 mRNA expression and secretion of IL-8 by an endometrial cancer cell line (Ishikawa) using the reverse transcriptase-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) methods, respectively.Entities:
Mesh：
Substances：
Year: 1994 PMID： 7738906 DOI： 10.1016/0165-0378(94)90001-9
Source DB: PubMed Journal: J Reprod Immunol ISSN： 0165-0378 Impact factor: 4.054