Literature DB >> 7738145

Analysis of enterovirus genotypes using single-strand conformation polymorphisms of polymerase chain reaction products.

S Fujioka1, H Koide, Y Kitaura, H Duguchi, K Kawamura.   

Abstract

Enterovirus genotypes were identified rapidly by reverse (RT-PCR) followed by single-strand conformation polymorphism (SSCP) analysis. The primer pair was chosen from the highly conserved sequence at the 5' non-coding region of enterovirus genomes. RT-PCR amplified a 154 bp sequence in all samples from 14 serotypes of enteroviruses, including group A and B Coxsackie viruses, echoviruses and polioviruses. SSCP analysis of these products revealed different electrophoretic profiles. Thus, SSCP analysis will be useful for differentiating the genotypes of enteroviruses, and may be applicable for rapid diagnosis of enteroviral infection.

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Year:  1995        PMID： 7738145     DOI： 10.1016/0166-0934(94)00112-t

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  7 in total

Review 1.  Methods for subtyping and molecular comparison of human viral genomes.

Authors:  M Arens
Journal:  Clin Microbiol Rev       Date:  1999-10       Impact factor: 26.132

2.  Molecular detection and identification of an enterovirus during an outbreak of aseptic meningitis.

Authors:  N Siafakas; A Georgopoulou; P Markoulatos; N Spyrou; G Stanway
Journal:  J Clin Lab Anal       Date:  2001       Impact factor: 2.352

3.  Use of base excision sequence scanning for detection of genetic variations in St. Louis encephalitis virus isolates.

Authors:  R N Charrel; N Lévy; R B Tesh; L J Chandler
Journal:  J Clin Microbiol       Date:  1999-06       Impact factor: 5.948

4.  Applications of polymerase chain reaction-single stranded conformational polymorphism to microbiology.

Authors:  J R Kerr; M D Curran
Journal:  Clin Mol Pathol       Date:  1996-12

5.  Differentiation and characterization of enteroviruses by computer-assisted viral protein fingerprinting.

Authors:  D T Holland; J Senne; C R Peter; C Urmenta; J D Connor
Journal:  J Clin Microbiol       Date:  1998-06       Impact factor: 5.948

Review 6.  Molecular typing of enteroviruses: current status and future requirements. The European Union Concerted Action on Virus Meningitis and Encephalitis.

Authors:  P Muir; U Kämmerer; K Korn; M N Mulders; T Pöyry; B Weissbrich; R Kandolf; G M Cleator; A M van Loon
Journal:  Clin Microbiol Rev       Date:  1998-01       Impact factor: 26.132

7.  Variability in molecular typing of Coxsackie A viruses by RFLP analysis and sequencing.

Authors:  Nikolaos Siafakas; Panayotis Markoulatos; Glyn Stanway
Journal:  J Clin Lab Anal       Date:  2002       Impact factor: 2.352
  7 in total

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