K L Meert1, M Long, J Kaplan, A P Sarnaik. 1. Department of Pediatrics, Children's Hospital of Michigan, Wayne State University School of Medicine, Detroit 48201, USA.
Abstract
OBJECTIVE: To investigate cellular and humoral immunity in children immediately after severe head injury and during the early recovery period. DESIGN: Prospective, observational study with factorial design. SETTING: Pediatric ICU of a university teaching hospital. PATIENTS: Fifteen children (median age 9.6 yrs, range 1.7 to 18) with head injury and Glasgow Coma Score of < or = 7. INTERVENTIONS: None. MEASUREMENTS AND MAIN RESULTS: Skin testing with seven standard antigens was performed and blood samples were obtained for the following measurements: total lymphocyte count and subsets; proliferative response to phytohemagglutinin, concanavalin A, and pokeweed mitogen; and immunoglobulin concentrations on days 1, 7, and 14 and 3 months after injury. The effect of patient plasma on phytohemagglutinin-induced proliferative responses of normal donor lymphocytes was also assessed at these times. Anergy was present in 71% of patients on day 1, 54% of patients on day 7, 31% of patients on day 14, and 18% of patients at 3 months. Total, helper, and suppressor T-cell counts were decreased on day 1, and the T-cell response to phytohemagglutinin was decreased on days 1, 7, and 14 compared with values at 3 months. B-cell counts were increased on day 1, followed by an increase in serum immunoglobulin concentrations 1 to 2 wks later. The B-cell response to pokeweed mitogen was unchanged over the 3-month study period. The phytohemagglutinin responses of normal donor lymphocytes were decreased when incubated with patient plasma obtained on day 7 after injury. CONCLUSIONS: Severe head injury in children is associated with depressed cell-mediated immunity. Plasma immunosuppressive factors may contribute to T-cell dysfunction.
OBJECTIVE: To investigate cellular and humoral immunity in children immediately after severe head injury and during the early recovery period. DESIGN: Prospective, observational study with factorial design. SETTING: Pediatric ICU of a university teaching hospital. PATIENTS: Fifteen children (median age 9.6 yrs, range 1.7 to 18) with head injury and Glasgow Coma Score of < or = 7. INTERVENTIONS: None. MEASUREMENTS AND MAIN RESULTS: Skin testing with seven standard antigens was performed and blood samples were obtained for the following measurements: total lymphocyte count and subsets; proliferative response to phytohemagglutinin, concanavalin A, and pokeweed mitogen; and immunoglobulin concentrations on days 1, 7, and 14 and 3 months after injury. The effect of patient plasma on phytohemagglutinin-induced proliferative responses of normal donor lymphocytes was also assessed at these times. Anergy was present in 71% of patients on day 1, 54% of patients on day 7, 31% of patients on day 14, and 18% of patients at 3 months. Total, helper, and suppressor T-cell counts were decreased on day 1, and the T-cell response to phytohemagglutinin was decreased on days 1, 7, and 14 compared with values at 3 months. B-cell counts were increased on day 1, followed by an increase in serum immunoglobulin concentrations 1 to 2 wks later. The B-cell response to pokeweed mitogen was unchanged over the 3-month study period. The phytohemagglutinin responses of normal donor lymphocytes were decreased when incubated with patient plasma obtained on day 7 after injury. CONCLUSIONS: Severe head injury in children is associated with depressed cell-mediated immunity. Plasma immunosuppressive factors may contribute to T-cell dysfunction.
Authors: Sebastian Arts; Erik J van Lindert; Rene Aquarius; Ronald H M A Bartels; Hieronymus D Boogaarts Journal: Acta Neurochir (Wien) Date: 2021-01-02 Impact factor: 2.216
Authors: Joseph A Carcillo; J Michael Dean; Richard Holubkov; John Berger; Kathleen L Meert; Kanwaljeet J S Anand; Jerry Zimmerman; Christopher J Newth; Rick Harrison; Jeri Burr; Douglas F Willson; Carol Nicholson; Michael J Bell; Robert A Berg; Thomas P Shanley; Sabrina M Heidemann; Heidi Dalton; Tammara L Jenkins; Allan Doctor; Angie Webster Journal: Pediatr Infect Dis J Date: 2016-11 Impact factor: 2.129
Authors: Joseph A Carcillo; J Michael Dean; Richard Holubkov; John Berger; Kathleen L Meert; Kanwaljeet J S Anand; Jerry J Zimmerman; Christopher J L Newth; Rick Harrison; Jeri Burr; Douglas F Willson; Carol Nicholson; Michael J Bell; Robert A Berg; Thomas P Shanley; Sabrina M Heidemann; Heidi Dalton; Tammara L Jenkins; Allan Doctor; Angie Webster; Robert F Tamburro Journal: JPEN J Parenter Enteral Nutr Date: 2016-09-22 Impact factor: 4.016