BACKGROUND: Glutamine is an important nutrient for the small intestine. Beneficial effects of glutamine could be related to restoration of optimal intestinal barrier functions. METHODS: Thirty-eight Sprague-Dawley rats were allocated to three main groups. Experimental groups (n = 22) were malnourished and laparotomized. Sham groups (n = 11) were laparotomized without prior malnutrition. These groups were refed with or without oral glutamine for 3 days. The control group (n = 5) was given chow. Permeability was assessed by the 6-h urinary recovery of orally given polyethylene glycols, PEG 400/1000. Mucosal proliferation was estimated by DNA content and 1-h incorporation of 3H-thymidine intravenously. RESULTS: In the malnourished groups glutamine resulted in higher thymidine incorporation (p < 0.05) and better absorption of small PEG molecules (p < 0.05). CONCLUSION: The effects of oral glutamine on permeability after malnourishment and laparotomy are proposed to be related to an increase in absorptive area.
BACKGROUND:Glutamine is an important nutrient for the small intestine. Beneficial effects of glutamine could be related to restoration of optimal intestinal barrier functions. METHODS: Thirty-eight Sprague-Dawley rats were allocated to three main groups. Experimental groups (n = 22) were malnourished and laparotomized. Sham groups (n = 11) were laparotomized without prior malnutrition. These groups were refed with or without oral glutamine for 3 days. The control group (n = 5) was given chow. Permeability was assessed by the 6-h urinary recovery of orally given polyethylene glycols, PEG 400/1000. Mucosal proliferation was estimated by DNA content and 1-h incorporation of 3H-thymidine intravenously. RESULTS: In the malnourished groups glutamine resulted in higher thymidine incorporation (p < 0.05) and better absorption of small PEG molecules (p < 0.05). CONCLUSION: The effects of oral glutamine on permeability after malnourishment and laparotomy are proposed to be related to an increase in absorptive area.
Authors: I Vázquez; I A Gómez-de-Segura; A G Grande; A Escribano; P González-Gancedo; A Gómez; R Díez; E De Miguel Journal: Dig Dis Sci Date: 1999-11 Impact factor: 3.199
Authors: Priscilla M Ueno; Reinaldo B Oriá; Elizabeth A Maier; Marjorie Guedes; Orleancio G de Azevedo; David Wu; Tara Willson; Simon P Hogan; Aldo A M Lima; Richard L Guerrant; D Brent Polk; Lee A Denson; Sean R Moore Journal: Am J Physiol Gastrointest Liver Physiol Date: 2011-07-28 Impact factor: 4.052
Authors: Reinaldo B Oriá; Peter D Patrick; James A Blackman; Aldo A M Lima; Richard L Guerrant Journal: Med Hypotheses Date: 2006-11-13 Impact factor: 1.538