| Literature DB >> 7730804 |
F Davidson1, P Simmonds, J C Ferguson, L M Jarvis, B C Dow, E A Follett, C R Seed, T Krusius, C Lin, G A Medgyesi.
Abstract
A method is described for identifying different genotypes of hepatitis C virus (HCV) by restriction endonuclease cleavage of sequences amplified by PCR from the 5' non-coding region. Using the enzymes HaeIII-RsaI and HinfI-MvaI, followed by cleavage with BstU1 or ScrFI, it was possible to identify and distinguish HCV genotypes 1a, 1b, 2a, 2b, 3a, 3b, 4, 5 and 6. The method was used to investigate the prevalence of these genotypes in 723 blood donors in 15 countries, the largest survey to date, and one which covered a wide range of geographical regions (Europe, America, Africa and Asia). These results, combined with a review of the existing literature, indicate the existence of several distinct regional patterns of HCV genotype distribution, and provide the framework for future detailed epidemiological investigations of HCV transmission.Entities:
Mesh：
Year: 1995 PMID： 7730804 DOI： 10.1099/0022-1317-76-5-1197
Source DB: PubMed Journal: J Gen Virol ISSN： 0022-1317 Impact factor: 3.891