Literature DB >> 7729688

The Rhizobium meliloti groELc locus is required for regulation of early nod genes by the transcription activator NodD.

J Ogawa1, S R Long.   

Abstract

The molecular chaperones related to GroEL (hsp60, cpn60) interact with partially folded proteins and appear to assist them to attain active and correctly folded conformation. They are required for cell viability but are probably more important for some processes than for others. Through a random genetic search to find loci that are required for expression of the Rhizobium meliloti nod (nodulation) genes, we isolated a mutant (B4) defective in luteolin-dependent activation of nod gene expression, and found it carries a Tn5 insertion within a chromosomal groEL gene (groELc) located just downstream of a groESc gene. The groELc mutation affected activity of three related LysR-type activator proteins NodD1, NodD3, and SyrM; on plants, the mutants formed nodules late, and the nodules were Fix-. Hybridization and protein expression analysis show that a similar groESL locus (groESLa) maps to the Rm1021 megaplasmid pSyma. Southern blot analysis revealed additional, but less closely related sequences hybridizing to groELc and groESc probes elsewhere in the R. meliloti genome. Clones of groESLc and groESLa can each restore robust phage lambda growth on an Escherichia coli groE mutant. Likewise each clone can complement all of the phenotypes observed for B4 mutants; thus, the two appear to be functionally equivalent if expression is controlled. We determined that groELc is required for normal DNA binding of the NodD target sequence in R. meliloti. GroEL coimmunopurifies with NodD1 from R. meliloti, which suggests a direct physical association between these proteins. GroEL is thus probably involved in the folding or assembly of transcriptionally active NodD.

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Year:  1995        PMID: 7729688     DOI: 10.1101/gad.9.6.714

Source DB:  PubMed          Journal:  Genes Dev        ISSN: 0890-9369            Impact factor:   11.361


  30 in total

1.  A chimeric prokaryotic ancestry of mitochondria and primitive eukaryotes.

Authors:  S Karlin; L Brocchieri; J Mrázek; A M Campbell; A M Spormann
Journal:  Proc Natl Acad Sci U S A       Date:  1999-08-03       Impact factor: 11.205

2.  Megaplasmid pRme2011a of Sinorhizobium meliloti is not required for viability.

Authors:  I J Oresnik; S L Liu; C K Yost; M F Hynes
Journal:  J Bacteriol       Date:  2000-06       Impact factor: 3.490

3.  High-resolution physical map of the Sinorhizobium meliloti 1021 pSyma megaplasmid.

Authors:  F Barloy-Hubler; D Capela; M J Barnett; S Kalman; N A Federspiel; S R Long; F Galibert
Journal:  J Bacteriol       Date:  2000-02       Impact factor: 3.490

Review 4.  Alpha-crystallin-type heat shock proteins: socializing minichaperones in the context of a multichaperone network.

Authors:  Franz Narberhaus
Journal:  Microbiol Mol Biol Rev       Date:  2002-03       Impact factor: 11.056

5.  Heat shock protein 60 sequence comparisons: duplications, lateral transfer, and mitochondrial evolution.

Authors:  S Karlin; L Brocchieri
Journal:  Proc Natl Acad Sci U S A       Date:  2000-10-10       Impact factor: 11.205

6.  Development of Sinorhizobium meliloti pilot macroarrays for transcriptome analysis.

Authors:  Hélène Bergès; Emmanuelle Lauber; Carine Liebe; Jacques Batut; Daniel Kahn; Frans J de Bruijn; Frédéric Ampe
Journal:  Appl Environ Microbiol       Date:  2003-02       Impact factor: 4.792

7.  Isolation and characterization of mutant Sinorhizobium meliloti NodD1 proteins with altered responses to luteolin.

Authors:  Melicent C Peck; Robert F Fisher; Robert Bliss; Sharon R Long
Journal:  J Bacteriol       Date:  2013-06-14       Impact factor: 3.490

Review 8.  Detection of and response to signals involved in host-microbe interactions by plant-associated bacteria.

Authors:  Anja Brencic; Stephen C Winans
Journal:  Microbiol Mol Biol Rev       Date:  2005-03       Impact factor: 11.056

9.  Cloning and characterization of the sigA gene encoding the major sigma subunit of Rhizobium meliloti.

Authors:  B G Rushing; S R Long
Journal:  J Bacteriol       Date:  1995-12       Impact factor: 3.490

10.  Proteomic alterations explain phenotypic changes in Sinorhizobium meliloti lacking the RNA chaperone Hfq.

Authors:  Lise Barra-Bily; Catherine Fontenelle; Gwenael Jan; Maud Flechard; Annie Trautwetter; Shree P Pandey; Graham C Walker; Carlos Blanco
Journal:  J Bacteriol       Date:  2010-01-15       Impact factor: 3.490

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