| Literature DB >> 7729556 |
V Ramesh1, Y Z Xu, G C Roberts.
Abstract
A convenient and economical method is described for the site-specific 15N-labelling of the 4-amino group of individual cytidine residues in oligonucleotides. This is applied to a 20 base-pair oligonucleotide corresponding to the trp operator; this oligonucleotide and its complex with the E. coli trp repressor are studied by NMR, using 1H-15N HMQC and 15N-edited 1H-1H NOESY experiments.Entities:
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Year: 1995 PMID： 7729556 DOI： 10.1016/0014-5793(95)00226-y
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124