Literature DB >> 7729500

Complex formation between gamma-immunoglobulin and calmodulin in calcium-free conditions.

Y Takeuchi1, P J Birckbichler, M K Patterson.   

Abstract

We show that gamma-immunoglobulin (IgG) binds calmodulin (CaM) in a Ca(2+)-independent manner, with Kd value of (1.7 +/- 0.5) x 10(-7) M. A single IgG molecule maximally bound 10 CaM molecules. The binding is to the heavy chain or Fab portion, but not the Fc portion, of the IgG molecules. Ca2+ greatly diminished the interaction between IgG and CaM, with IC50 = 8-9 microM. These data give a novel insight into protein-protein interactions.

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Year:  1995        PMID： 7729500     DOI： 10.1007/bf01928891

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  12 in total

Review 1.  Calcium oscillations.

Authors:  M J Berridge
Journal:  J Biol Chem       Date:  1990-06-15       Impact factor: 5.157

2.  Identification and characterization of the calmodulin-binding domain of neuromodulin, a neurospecific calmodulin-binding protein.

Authors:  K A Alexander; B T Wakim; G S Doyle; K A Walsh; D R Storm
Journal:  J Biol Chem       Date:  1988-06-05       Impact factor: 5.157

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  Catalytic properties of a calmodulin-regulated transglutaminase from human platelet and chicken gizzard.

Authors:  E G Puszkin; V Raghuraman
Journal:  J Biol Chem       Date:  1985-12-15       Impact factor: 5.157

5.  Can calmodulin function without binding calcium?

Authors:  J R Geiser; D van Tuinen; S E Brockerhoff; M M Neff; T N Davis
Journal:  Cell       Date:  1991-06-14       Impact factor: 41.582

6.  Phosphorylase kinase phosphorylates the calmodulin-binding regulatory regions of neuronal tissue-specific proteins B-50 (GAP-43) and neurogranin.

Authors:  H K Paudel; H Zwiers; J H Wang
Journal:  J Biol Chem       Date:  1993-03-25       Impact factor: 5.157

7.  Measurement of protein using bicinchoninic acid.

Authors:  P K Smith; R I Krohn; G T Hermanson; A K Mallia; F H Gartner; M D Provenzano; E K Fujimoto; N M Goeke; B J Olson; D C Klenk
Journal:  Anal Biochem       Date:  1985-10       Impact factor: 3.365

8.  Regulation of calmodulin binding to P-57. A neurospecific calmodulin binding protein.

Authors:  K A Alexander; B M Cimler; K E Meier; D R Storm
Journal:  J Biol Chem       Date:  1987-05-05       Impact factor: 5.157

9.  Purification and characterization of a brain-specific protein kinase C substrate, neurogranin (p17). Identification of a consensus amino acid sequence between neurogranin and neuromodulin (GAP43) that corresponds to the protein kinase C phosphorylation site and the calmodulin-binding domain.

Authors:  J Baudier; J C Deloulme; A Van Dorsselaer; D Black; H W Matthes
Journal:  J Biol Chem       Date:  1991-01-05       Impact factor: 5.157

10.  Purification of a novel calmodulin binding protein from bovine cerebral cortex membranes.

Authors:  T J Andreasen; C W Luetje; W Heideman; D R Storm
Journal:  Biochemistry       Date:  1983-09-27       Impact factor: 3.162
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