Literature DB >> 7729484

Trichinella spiralis: cloning and characterisation of two repetitive DNA sequences.

B Connolly1, L J Ingram, D F Smith.   

Abstract

Digestion of genomic DNA of Trichinella spiralis with EcoRI reveals the presence of several families of repetitive DNA. Two small repeats of 516 and 604 bp, respectively, have been cloned and sequenced. The two repeats are related to each other and to a 1.1-kb repetitive DNA. Similar sequences are not detected in other species by hybridisation. There are about 270 copies of each small repeat per haploid genome and these are organised in large arrays of > or = 50 kb. The basic repetitive unit appears to contain one copy of each repeat. This repetitive DNA is transcribed in muscle-stage larvae although it is not protein coding.

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Year:  1995        PMID: 7729484     DOI: 10.1006/expr.1995.1061

Source DB:  PubMed          Journal:  Exp Parasitol        ISSN: 0014-4894            Impact factor:   2.011


  3 in total

1.  Differential diagnosis of Taenia saginata and Taenia solium infection by PCR.

Authors:  L M González; E Montero; L J Harrison; R M Parkhouse; T Garate
Journal:  J Clin Microbiol       Date:  2000-02       Impact factor: 5.948

2.  Molecular cloning and characterization of an antigenic protein with a repeating region from Clonorchis sinensis.

Authors:  T Y Kim; S Y Kang; I Y Ahn; S Y Cho; S J Hong
Journal:  Korean J Parasitol       Date:  2001-03       Impact factor: 1.341

3.  Spliced leader trans-splicing in the nematode Trichinella spiralis uses highly polymorphic, noncanonical spliced leaders.

Authors:  Jonathan Pettitt; Berndt Müller; Ian Stansfield; Bernadette Connolly
Journal:  RNA       Date:  2008-02-06       Impact factor: 4.942

  3 in total

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