R Lundblad1, K E Giercksky. 1. Department of Surgical Oncology, Norwegian Radium Hospital, Oslo.
Abstract
OBJECTIVE: To define the profiles of endothelin (ET), both big ET and active 21 amino acid ET (ET 1-21) in the plasma and peritoneal cavity of rats with peritonitis. DESIGN: Open laboratory study. SETTING: University hospital, Norway. MATERIAL: 170 adult male Wistar rats. INTERVENTIONS: Lethal peritonitis was induced by making a 4 mm caecal perforation. MAIN OUTCOME MEASURES: Mortality, together with concentrations of total ET, ET 1-21 (measured by two radioimmunoassays), bacteria, endotoxin, tumor necrosis factor (TNF), interleukin 6 (IL-6), and lactate at baseline, two hours, and then four-hourly intervals for 24 hours. RESULTS: ET reached its maximum at 8 hours, and had returned to baseline after 24 hours. In the first phase of septicaemia there was more big ET than ET 1-21, but the proportions had equalised by 8 hours. There were higher concentrations of both big ET and ET 1-21 in peritoneal fluid than in plasma. CONCLUSIONS: In rats with peritonitis the profiles of big ET and ET 1-21 closely followed mortality, and blood concentrations of bacteria, endotoxin, TNF, IL-6, and lactate.
OBJECTIVE: To define the profiles of endothelin (ET), both big ET and active 21 amino acid ET (ET 1-21) in the plasma and peritoneal cavity of rats with peritonitis. DESIGN: Open laboratory study. SETTING: University hospital, Norway. MATERIAL: 170 adult male Wistar rats. INTERVENTIONS: Lethal peritonitis was induced by making a 4 mm caecal perforation. MAIN OUTCOME MEASURES: Mortality, together with concentrations of total ET, ET 1-21 (measured by two radioimmunoassays), bacteria, endotoxin, tumor necrosis factor (TNF), interleukin 6 (IL-6), and lactate at baseline, two hours, and then four-hourly intervals for 24 hours. RESULTS: ET reached its maximum at 8 hours, and had returned to baseline after 24 hours. In the first phase of septicaemia there was more big ET than ET 1-21, but the proportions had equalised by 8 hours. There were higher concentrations of both big ET and ET 1-21 in peritoneal fluid than in plasma. CONCLUSIONS: In rats with peritonitis the profiles of big ET and ET 1-21 closely followed mortality, and blood concentrations of bacteria, endotoxin, TNF, IL-6, and lactate.
Authors: Lars A Schneider; Susan M Schlenner; Thorsten B Feyerabend; Markus Wunderlin; Hans-Reimer Rodewald Journal: J Exp Med Date: 2007-10-08 Impact factor: 14.307