| Literature DB >> 7721103 |
T McGonigal1, P Bodelle, C Schopp, A V Sarthy.
Abstract
The construction of a human DNA library in a centromere-based circular yeast plasmid is described. The vector contains the yeast CEN3 sequence, the URA3 gene for propagation in yeast and a hygromycin-resistance gene (HyR) for selection in mammalian cells. The library consists of 64,000 members with an average insert size of 150 kb, with some members containing inserts of > 1 Mb. We calculate that the library contains three human genome equivalents of DNA. Clones can be identified by a PCR-based screening of DNA pools from individual colonies that have been stored in microtiter wells.Entities:
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Year: 1995 PMID： 7721103 DOI： 10.1016/0378-1119(94)00887-x
Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688