Literature DB >> 7721089

Cloning and transient expression of genes encoding the human alpha 4 and beta 2 neuronal nicotinic acetylcholine receptor (nAChR) subunits.

L M Monteggia1, M Gopalakrishnan, E Touma, K B Idler, N Nash, S P Arneric, J P Sullivan, T Giordano.   

Abstract

Partial cDNA clones generated by RT-PCR were used as probes to clone the cDNAs encoding the human alpha 4 and beta 2 neuronal nicotinic acetylcholine receptor (nAChR) subunits. The 2.1-kb alpha 4 cDNA shows 84 and 76% identity to the rat and chicken cDNA sequences, respectively. The deduced amino-acid sequence shares 89 and 84% similarity, respectively, with the corresponding rat and chicken proteins, with most of the divergence occurring in the cytoplasmic domain. The 1721-nucleotide beta 2 sequence was identical to the human beta 2 sequence previously reported. Transfection of the alpha 4 and beta 2 clones into HEK293 cells resulted in the formation of binding sites that display high affinity towards [3H] cytisine, a characteristic of the alpha 4 beta 2 subtype produced in vivo.

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Year:  1995        PMID： 7721089     DOI： 10.1016/0378-1119(94)00914-e

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  7 in total

1.  Comparative structure of human neuronal alpha 2-alpha 7 and beta 2-beta 4 nicotinic acetylcholine receptor subunits and functional expression of the alpha 2, alpha 3, alpha 4, alpha 7, beta 2, and beta 4 subunits.

Authors:  K J Elliott; S B Ellis; K J Berckhan; A Urrutia; L E Chavez-Noriega; E C Johnson; G Veliçelebi; M M Harpold
Journal:  J Mol Neurosci       Date:  1996       Impact factor: 3.444

2.  CHRNB2 is the second acetylcholine receptor subunit associated with autosomal dominant nocturnal frontal lobe epilepsy.

Authors:  H A Phillips; I Favre; M Kirkpatrick; S M Zuberi; D Goudie; S E Heron; I E Scheffer; G R Sutherland; S F Berkovic; D Bertrand; J C Mulley
Journal:  Am J Hum Genet       Date:  2000-12-05       Impact factor: 11.025

3.  Characterization of human alpha4beta2 neuronal nicotinic receptors stably expressed in SH-EP1 cells.

Authors:  M A Pacheco; T E Pastoor; R J Lukas; L Wecker
Journal:  Neurochem Res       Date:  2001-06       Impact factor: 3.996

4.  Human alpha4beta2 neuronal nicotinic acetylcholine receptor in HEK 293 cells: A patch-clamp study.

Authors:  B Buisson; M Gopalakrishnan; S P Arneric; J P Sullivan; D Bertrand
Journal:  J Neurosci       Date:  1996-12-15       Impact factor: 6.167

5.  Homology modeling and molecular dynamics simulations of transmembrane domain structure of human neuronal nicotinic acetylcholine receptor.

Authors:  Alexander C Saladino; Yan Xu; Pei Tang
Journal:  Biophys J       Date:  2004-12-01       Impact factor: 4.033

6.  Five ADNFLE mutations reduce the Ca2+ dependence of the mammalian alpha4beta2 acetylcholine response.

Authors:  Nivalda Rodrigues-Pinguet; Li Jia; Maureen Li; Antonio Figl; Alwin Klaassen; Anthony Truong; Henry A Lester; Bruce N Cohen
Journal:  J Physiol       Date:  2003-05-16       Impact factor: 5.182

7.  Rapid Akt activation by nicotine and a tobacco carcinogen modulates the phenotype of normal human airway epithelial cells.

Authors:  Kip A West; John Brognard; Amy S Clark; Ilona R Linnoila; Xiaowei Yang; Sandra M Swain; Curtis Harris; Steven Belinsky; Phillip A Dennis
Journal:  J Clin Invest       Date:  2003-01       Impact factor: 14.808
  7 in total

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