Literature DB >> 7720853

Purification of the hepatic glycogen-associated form of protein phosphatase-1 by microcystin-Sepharose affinity chromatography.

G Moorhead1, C MacKintosh, N Morrice, P Cohen.   

Abstract

The form of protein phosphatase-1 associated with hepatic glycogen (PP1G) was purified to near homogeneity from rat liver by affinity chromatography on microcystin-Sepharose and gel-filtration. The enzyme is a heterodimer consisting of the catalytic subunit of PP1 (the alpha and beta isoforms) complexed to a 33 kDa glycogen-binding (GL) subunit. The GL subunit binds phosphorylase a with high affinity, and is responsible for the enhanced dephosphorylation of glycogen synthase by PP1G and its allosteric inhibition by phosphorylase a.

Entities:  

Mesh：

Substances：

Year:  1995        PMID： 7720853     DOI： 10.1016/0014-5793(95)00197-h

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  18 in total

1.  Mutations of the serine phosphorylated in the protein phosphatase-1-binding motif in the skeletal muscle glycogen-targeting subunit.

Authors:  J Liu; J Wu; C Oliver; S Shenolikar; D L Brautigan
Journal:  Biochem J       Date:  2000-02-15       Impact factor: 3.857

Review 2.  Specific features of glycogen metabolism in the liver.

Authors:  M Bollen; S Keppens; W Stalmans
Journal:  Biochem J       Date:  1998-11-15       Impact factor: 3.857

3.  Identification of the separate domains in the hepatic glycogen-targeting subunit of protein phosphatase 1 that interact with phosphorylase a, glycogen and protein phosphatase 1.

Authors:  C G Armstrong; M J Doherty; P T Cohen
Journal:  Biochem J       Date:  1998-12-15       Impact factor: 3.857

4.  Loss of the hepatic glycogen-binding subunit (GL) of protein phosphatase 1 underlies deficient glycogen synthesis in insulin-dependent diabetic rats and in adrenalectomized starved rats.

Authors:  M J Doherty; J Cadefau; W Stalmans; M Bollen; P T Cohen
Journal:  Biochem J       Date:  1998-07-15       Impact factor: 3.857

5.  The level of the glycogen targetting regulatory subunit R5 of protein phosphatase 1 is decreased in the livers of insulin-dependent diabetic rats and starved rats.

Authors:  G J Browne; M Delibegovic; S Keppens; W Stalmans; P T Cohen
Journal:  Biochem J       Date:  2001-12-01       Impact factor: 3.857

Review 6.  Glycogen and its metabolism: some new developments and old themes.

Authors:  Peter J Roach; Anna A Depaoli-Roach; Thomas D Hurley; Vincent S Tagliabracci
Journal:  Biochem J       Date:  2012-02-01       Impact factor: 3.857

7.  Hepatic protein phosphatase 1 regulatory subunit 3B (Ppp1r3b) promotes hepatic glycogen synthesis and thereby regulates fasting energy homeostasis.

Authors:  Minal B Mehta; Swapnil V Shewale; Raymond N Sequeira; John S Millar; Nicholas J Hand; Daniel J Rader
Journal:  J Biol Chem       Date:  2017-05-04       Impact factor: 5.157

8.  Structural basis for the recognition of regulatory subunits by the catalytic subunit of protein phosphatase 1.

Authors:  M P Egloff; D F Johnson; G Moorhead; P T Cohen; P Cohen; D Barford
Journal:  EMBO J       Date:  1997-04-15       Impact factor: 11.598

9.  PTG gene deletion causes impaired glycogen synthesis and developmental insulin resistance.

Authors:  Sean M Crosson; Ahmir Khan; John Printen; Jeffrey E Pessin; Alan R Saltiel
Journal:  J Clin Invest       Date:  2003-05       Impact factor: 14.808

10.  Differential regulation of glycogenolysis by mutant protein phosphatase-1 glycogen-targeting subunits.

Authors:  Arpad M Danos; Senad Osmanovic; Matthew J Brady
Journal:  J Biol Chem       Date:  2009-06-01       Impact factor: 5.157
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.