OBJECTIVE: To determine whether heparin anticoagulants used for analysis of whole blood ionized calcium would influence the measurement of ionized magnesium. METHODS: The effects of zinc heparin, lithium heparin, and electrolyte-balanced heparin on the simultaneous measurement of ionized magnesium and ionized calcium in serum were determined using ion selective electrodes. RESULTS: Time-dependent biases in ionized magnesium and calcium concentrations were apparent with zinc heparin but not with lithium or electrolyte-balanced heparin. Ionized magnesium and calcium concentrations were more significantly influenced by volume-dependent changes in zinc heparin potency than with lithium or electrolyte-balanced heparin. CONCLUSION: Zinc heparin produces a significant positive bias in the simultaneous determination of ionized magnesium and ionized calcium concentrations.
OBJECTIVE: To determine whether heparin anticoagulants used for analysis of whole blood ionizedcalcium would influence the measurement of ionizedmagnesium. METHODS: The effects of zinc heparin, lithium heparin, and electrolyte-balanced heparin on the simultaneous measurement of ionizedmagnesium and ionizedcalcium in serum were determined using ion selective electrodes. RESULTS: Time-dependent biases in ionizedmagnesium and calcium concentrations were apparent with zinc heparin but not with lithium or electrolyte-balanced heparin. Ionizedmagnesium and calcium concentrations were more significantly influenced by volume-dependent changes in zinc heparin potency than with lithium or electrolyte-balanced heparin. CONCLUSION:Zinc heparin produces a significant positive bias in the simultaneous determination of ionizedmagnesium and ionizedcalcium concentrations.
Authors: Janlee A Jensen; Angela K Brice; Jessica H Bagel; Angela M Mexas; Sea Young Yoon; John H Wolfe Journal: Comp Med Date: 2013-04 Impact factor: 0.982