Activation of murine macrophages by hydrogen peroxide.

Hydrogen peroxide at concentrations from 0.1 to 20 microM enhances phagocytosis and oxidative burst of murine peritoneal macrophages. The activation of these macrophage functions is paralleled by prolonged hyperpolarization and a transient increase in cytoplasmic free calcium concentration. All the effects are dose- and time-dependent. The results obtained for H2O2 are compared with those for a natural activator, peptide N-formyl-methionyl-leucyl-phenylalanine. The data demonstrate the ability of small doses of hydrogen peroxide to stimulate macrophages through the intracellular mechanisms of ion transduction.