Literature DB >> 7718231

Vaccinia virus transfection of hippocampal slice neurons.

D L Pettit1, T Koothan, D Liao, R Malinow.   

Abstract

Here we describe a technique that uses a recombinant vaccinia virus to transfect neurons in rat hippocampal slices. This technique allows the use of molecular biological manipulations on neuronal tissue while maintaining intact synaptic function. This method should be useful in testing specific hypotheses regarding the role of synaptic proteins.

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Year:  1995        PMID： 7718231     DOI： 10.1016/0896-6273(95)90212-0

Source DB:  PubMed          Journal:  Neuron        ISSN： 0896-6273            Impact factor:   17.173


  6 in total

1.  Characterization of promoter function and cell-type-specific expression from viral vectors in the nervous system.

Authors:  R L Smith; D L Traul; J Schaack; G H Clayton; K J Staley; C L Wilcox
Journal:  J Virol       Date:  2000-12       Impact factor: 5.103

2.  Suspended-drop electroporation for high-throughput delivery of biomolecules into cells.

Authors:  Emmanuel G Guignet; Tobias Meyer
Journal:  Nat Methods       Date:  2008-04-13       Impact factor: 28.547

3.  Virus-mediated gene transfer into hippocampal CA1 region restores long-term potentiation in brain-derived neurotrophic factor mutant mice.

Authors:  M Korte; O Griesbeck; C Gravel; P Carroll; V Staiger; H Thoenen; T Bonhoeffer
Journal:  Proc Natl Acad Sci U S A       Date:  1996-10-29       Impact factor: 11.205

4.  Introduction of green fluorescent protein (GFP) into hippocampal neurons through viral infection.

Authors:  Roberto Malinow; Yasunori Hayashi; Mirjana Maletic-Savatic; Shahid H Zaman; Jean-Christophe Poncer; Song-Hai Shi; José A Esteban; Pavel Osten; Ken Seidenman
Journal:  Cold Spring Harb Protoc       Date:  2010-04

Review 5.  Genetically engineered poxviruses for recombinant gene expression, vaccination, and safety.

Authors:  B Moss
Journal:  Proc Natl Acad Sci U S A       Date:  1996-10-15       Impact factor: 11.205

6.  Calcium-evoked dendritic exocytosis in cultured hippocampal neurons. Part II: mediation by calcium/calmodulin-dependent protein kinase II.

Authors:  M Maletic-Savatic; T Koothan; R Malinow
Journal:  J Neurosci       Date:  1998-09-01       Impact factor: 6.167
  6 in total

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