Characterization of synaptic connections between cortex and deep nuclei of the rat cerebellum in vitro.

Intracellular recordings were used to characterize the inhibitory synapses formed by Purkinje cells on neurons in the deep cerebellar nuclei of the rat. This work was performed on organotypic cerebellar cultures where functional connections between Purkinje cells and deep cerebellar neurons are formed de novo. After blocking ionotropic excitatory amino acid, and GABAA receptors with 6-cyano-7-nitro-quinoxaline-2,3-dione,D-2-amino-5-phosphonovalerate and bicuculline, respectively, the majority of deep cerebellar neurons fired spontaneously without accommodation. This tonic firing was linearly dependent on membrane potential and was abolished with hyperpolarization. Bath application of muscimol and baclofen reversibly hyperpolarized deep cerebellar nuclei cells. In the presence of excitatory amino acid receptor antagonists, field stimulation within the Purkinje cell layer induced monosynaptic inhibitory potentials in deep cerebellar neurons that were graded and completely blocked by bicuculline. Inhibitory potential amplitudes were not markedly reduced during fast repetitive stimulation of Purkinje cells, and the resulting hyperpolarization was not affected by the competitive GABAB receptor antagonist CGP 35348. A single inhibitory potential temporarily interrupted trains of action potentials induced in deep cerebellar cells by short depolarizing pulses. Trains of five inhibitory postsynaptic potentials, evoked at 20 Hz, induced a hyperpolarization which transiently blocked the spontaneous firing of deep cerebellar cells. The efficiency to block action potential discharges depended on the frequency of evoked inhibitory potentials. Bath application of bicuculline induced burst discharges in the control solution. When the excitatory amino acid receptors were pharmacologically blocked, bicuculline depolarized deep cerebellar neurons inducing sustained action potential discharges. In the presence of tetrodotoxin, bicuculline abolished miniature inhibitory postsynaptic potentials and resulted in a membrane depolarization of deep cerebellar cells. We conclude that deep cerebellar neurons isolated from synaptic inputs display a pacemaker-like activity. Although these neurons possess GABAA and GABAB receptors, we confirm that only GABAA receptors were involved in the generation of inhibitory postsynaptic potentials, even with high frequency stimulation. The amplitude of evoked inhibitory potentials was weakly frequency-dependent, thus allowing a powerful inhibition of the pacemaker-like activity by trains of evoked inhibitory postsynaptic potentials. Additionally, spontaneous and miniature inhibitory potentials control the excitability of deep cerebellar neurons by exerting a continuous hyperpolarizing tone.