| Literature DB >> 7714342 |
V Kouskoff1, K Signorelli, C Benoist, D Mathis.
Abstract
We describe a pair of cassette vectors that can be used to express rearranged T cell receptor genes in transgenic mice. Short DNA fragments containing rearranged V alpha and V beta segments are readily amplified from T cells and introduced between artificial cloning sites. Transgene-derived mRNAs are transcribed under the control of the natural TCR alpha and -beta promoter/enhancer elements. Using this vector, we have obtained transgenic mouse lines which display transgene-encoded TCR alpha and beta chains on a majority of T cells.Entities:
Mesh：
Substances：
Year: 1995 PMID： 7714342 DOI： 10.1016/0022-1759(95)00002-r
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303