Literature DB >> 7714047

Increased viral titers and enhanced reactivity of antibodies to the spike glycoprotein of murine coronavirus produced by infection at pH 6.

C Daniel1, A Lamarre, P J Talbot.   

Abstract

Infection of cell monolayers by murine coronavirus A59 at pH 6 rather than 7 yielded a ten-fold increase in the infectious titer and a remarkable enhancement of the reactivities of monoclonal and polyclonal antibodies against the spike glycoprotein in immunoblotting, immunoprecipitation and enzyme-linked immunosorbent assays. These observations are very useful for detecting antibodies against the S glycoprotein of coronaviruses and enhancing infectious titers.

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Year:  1994        PMID： 7714047      PMCID： PMC7119785          DOI： 10.1016/0166-0934(94)90180-5

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  19 in total

1.  Receptor for mouse hepatitis virus is a member of the carcinoembryonic antigen family of glycoproteins.

Authors:  R K Williams; G S Jiang; K V Holmes
Journal:  Proc Natl Acad Sci U S A       Date:  1991-07-01       Impact factor: 11.205

2.  Identification of an immunodominant linear neutralization domain on the S2 portion of the murine coronavirus spike glycoprotein and evidence that it forms part of complex tridimensional structure.

Authors:  C Daniel; R Anderson; M J Buchmeier; J O Fleming; W J Spaan; H Wege; P J Talbot
Journal:  J Virol       Date:  1993-03       Impact factor: 5.103

3.  Replication and plaque formation of mouse hepatitis virus (MHV-2) in mouse cell line DBT culture.

Authors:  N Hirano; K Fujiwara; S Hino; M Matumoto
Journal:  Arch Gesamte Virusforsch       Date:  1974

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Intracellular transport of recombinant coronavirus spike proteins: implications for virus assembly.

Authors:  H Vennema; L Heijnen; A Zijderveld; M C Horzinek; W J Spaan
Journal:  J Virol       Date:  1990-01       Impact factor: 5.103

6.  Hybridoma antibodies to the murine coronavirus JHM: characterization of epitopes on the peplomer protein (E2).

Authors:  H Wege; R Dörries; H Wege
Journal:  J Gen Virol       Date:  1984-11       Impact factor: 3.891

7.  A dot-immunobinding assay for monoclonal and other antibodies.

Authors:  R Hawkes; E Niday; J Gordon
Journal:  Anal Biochem       Date:  1982-01-01       Impact factor: 3.365

8.  Monoclonal antibodies to the peplomer glycoprotein of coronavirus mouse hepatitis virus identify two subunits and detect a conformational change in the subunit released under mild alkaline conditions.

Authors:  D G Weismiller; L S Sturman; M J Buchmeier; J O Fleming; K V Holmes
Journal:  J Virol       Date:  1990-06       Impact factor: 5.103

9.  Conformational change of the coronavirus peplomer glycoprotein at pH 8.0 and 37 degrees C correlates with virus aggregation and virus-induced cell fusion.

Authors:  L S Sturman; C S Ricard; K V Holmes
Journal:  J Virol       Date:  1990-06       Impact factor: 5.103

10.  Physico-chemical properties of murine hepatitis virus, strain A 59. Brief report.

Authors:  C Daniel; P J Talbot
Journal:  Arch Virol       Date:  1987       Impact factor: 2.574
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  1 in total

1.  Methylene blue in combination with sunlight as a low cost and effective disinfection method for coronavirus-contaminated PPE.

Authors:  Kevin A Vos; Paul M K Gordon; Belinda Heyne
Journal:  Am J Infect Control       Date:  2022-08       Impact factor: 4.303
  1 in total

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