Literature DB >> 7711100

Preparation of oligonucleotide-biotin conjugates with cleavable linkers.

G A Soukup1, R L Cerny, L J Maher.   

Abstract

A procedure is presented for preparing an oligonucleotide-biotin conjugate that is chemically cleavable through the reduction of a disulfide bond within the linker. Conjugation involves reaction of a primary amine with an N-hydroxysulfosuccinimide ester linked to biotin. The oligonucleotide can be liberated from streptavidin agarose containing immobilized conjugate under mild conditions (neutral pH, 50 mM dithiothreitol). This cleavable conjugate is useful for affinity purification applications.

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Year:  1995        PMID： 7711100     DOI： 10.1021/bc00031a017

Source DB:  PubMed          Journal:  Bioconjug Chem        ISSN： 1043-1802            Impact factor:   4.774


  4 in total

1.  Selection of genomic sequences that bind tightly to Ff gene 5 protein: primer-free genomic SELEX.

Authors:  Jin-Der Wen; Donald M Gray
Journal:  Nucleic Acids Res       Date:  2004-12-15       Impact factor: 16.971

2.  Photocleavable biotin phosphoramidite for 5'-end-labeling, affinity purification and phosphorylation of synthetic oligonucleotides.

Authors:  J Olejnik; E Krzymańska-Olejnik; K J Rothschild
Journal:  Nucleic Acids Res       Date:  1996-01-15       Impact factor: 16.971

3.  RNAs synthesized using photocleavable biotinylated nucleotides have dramatically improved catalytic efficiency.

Authors:  Yiling Luo; Nadukkudy V Eldho; Herman O Sintim; T Kwaku Dayie
Journal:  Nucleic Acids Res       Date:  2011-07-08       Impact factor: 16.971

4.  Analytical biochemistry of DNA--protein assemblies from crude cell extracts.

Authors:  Nadia Hégarat; Gildas Mouta Cardoso; Filippo Rusconi; Jean-Christophe Francois; Danièle Praseuth
Journal:  Nucleic Acids Res       Date:  2007-07-07       Impact factor: 16.971
  4 in total

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