Literature DB >> 7710281

Detection of Bacteroides fragilis endotoxin in amniotic fluid by counterimmunoelectrophoresis.

I Beckmann1, K de Graaff, F Meisel-Mikołajczyk, H C Wallenburg.   

Abstract

The ability of counter immunoelectrophoresis (CIE) to detect Bacteroides fragilis endotoxin in amniotic fluid in small concentrations was evaluated. A method was developed which, in combination with ultrafiltration, permits detection of B. fragilis endotoxin in amniotic fluid in a concentration of 40 ng/ml or more. The sensitivity threshold was reduced to 2 ng/ml by using a highly reactive IgG-fraction isolated from rabbit anti-B. fragilis IPL E 323 antiserum.

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Year:  1994        PMID: 7710281     DOI: 10.1007/bf00882769

Source DB:  PubMed          Journal:  Antonie Van Leeuwenhoek        ISSN: 0003-6072            Impact factor:   2.271


  15 in total

1.  Counter-current immunoelectrophoresis for the diagnosis of pneumococcal chest infection.

Authors:  M El-Refaie; C Dulake
Journal:  J Clin Pathol       Date:  1975-10       Impact factor: 3.411

2.  Countercurrent immunoelectrophoresis in the diagnosis of systemic diseases caused by Hemophilus infleunzae type b.

Authors:  D L Ingram; P Anderson; D H Smith
Journal:  J Pediatr       Date:  1972-12       Impact factor: 4.406

3.  A manual of quantitative immunoelectrophoresis. Methods and applications. 1. General remarks on principles, equipment, reagents and procedures.

Authors:  B Weeke
Journal:  Scand J Immunol Suppl       Date:  1973

4.  Detection of streptococcal antigen by counterimmunoelectrophoresis.

Authors:  R F Jacobs; T Yamauchi; K D Eisenach
Journal:  Am J Clin Pathol       Date:  1981-02       Impact factor: 2.493

5.  Coagglutination and counter immunoelectrophoresis in the rapid diagnosis of typhoid fever.

Authors:  N P Shetty; H Srinivasa; P Bhat
Journal:  Am J Clin Pathol       Date:  1985-07       Impact factor: 2.493

6.  Immunochemical characterization of the outer membrane complex of Bacteroides fragilis subspecies fragilis.

Authors:  D L Kasper; M W Seiler
Journal:  J Infect Dis       Date:  1975-10       Impact factor: 5.226

7.  Routine use of counterimmunoelectrophoresis for the detection of pneumococcal antigen in sputum.

Authors:  C H Ericsson; H O Hallander; A Rosen; A M Sjögren; I Sjögren
Journal:  Med Microbiol Immunol       Date:  1986       Impact factor: 3.402

8.  Countercurrent immunoelectrophoresis in the evaluation of childhood infections.

Authors:  P G Shackelford; J Campbell; R D Feigin
Journal:  J Pediatr       Date:  1974-10       Impact factor: 4.406

9.  Immunochemical investigations of antigens isolated from Bacteroides ovatus strain ATCC 8483.

Authors:  I Beckmann; J Paelinck; J Zuijderduijn; A Sawicka-Grzelak; F Meisel-Mikołajczyk
Journal:  Antonie Van Leeuwenhoek       Date:  1985       Impact factor: 2.271

10.  Detection of 2-keto-3-deoxyoctonate in endotoxins isolated from six reference strains of the Bacteroides fragilis group.

Authors:  I Beckmann; H G van Eijk; F Meisel-Mikołajczyk; H C Wallenburg
Journal:  Int J Biochem       Date:  1989
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