W D Davenport1, E R Allen. 1. Department of Oral Pathology, Louisiana State University Medical Center, Schools of Dentistry and Medicine, New Orleans 70119.
Abstract
BACKGROUND: The immunological role played by the avian bursa of Fabricius has been well established. Although numerous studies have also reported on the development and general morphology of this organ, some structure-function relationships still have not been fully explained. METHODS: Bursae from chickens at three developmental stages were removed and examined by scanning electron microscopy. Routine preparation was used as well as sonication (microdissection). Micrographs were used for qualitative morphological study and for quantitative morphometric analyses. RESULTS: Routine SEM observations were similar to those previously reported in the literature. Sonicated specimens allowed topographical study of various levels of surface erosion. Two types of surface cells were observed: typical absorptive epithelium and follicle-associated epithelial (FAE) cells. Erosion of the dome surface epithelium revealed basal lamina pores in the region over the subepithelial lymphoid follicles. These pores were present at hatching. Morphometric analysis of dome and pore areas revealed that the pore area decreases in relation to dome area with aging. CONCLUSIONS: Basal lamina pores may provide a communication route between the lymphoid follicles and the external environment via the FAE cells. Also, the close association between the FAE cells of the epithelial domes, the epithelial pores, the capillary complex of the previously described bursal--blood barrier, and the subepithelial lymphoid follicles could represent a morphological "pore complex" that matures early in posthatching development and may be related to the immunological function of the bursa.
BACKGROUND: The immunological role played by the avian bursa of Fabricius has been well established. Although numerous studies have also reported on the development and general morphology of this organ, some structure-function relationships still have not been fully explained. METHODS: Bursae from chickens at three developmental stages were removed and examined by scanning electron microscopy. Routine preparation was used as well as sonication (microdissection). Micrographs were used for qualitative morphological study and for quantitative morphometric analyses. RESULTS: Routine SEM observations were similar to those previously reported in the literature. Sonicated specimens allowed topographical study of various levels of surface erosion. Two types of surface cells were observed: typical absorptive epithelium and follicle-associated epithelial (FAE) cells. Erosion of the dome surface epithelium revealed basal lamina pores in the region over the subepithelial lymphoid follicles. These pores were present at hatching. Morphometric analysis of dome and pore areas revealed that the pore area decreases in relation to dome area with aging. CONCLUSIONS: Basal lamina pores may provide a communication route between the lymphoid follicles and the external environment via the FAE cells. Also, the close association between the FAE cells of the epithelial domes, the epithelial pores, the capillary complex of the previously described bursal--blood barrier, and the subepithelial lymphoid follicles could represent a morphological "pore complex" that matures early in posthatching development and may be related to the immunological function of the bursa.
Authors: Ryo Goitsuka; Chen-Lo H Chen; Lesley Benyon; Yusuke Asano; Daisuke Kitamura; Max D Cooper Journal: Proc Natl Acad Sci U S A Date: 2007-09-07 Impact factor: 11.205