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Literature DB >> 7708487

Sse8647I, a new type II restriction endonuclease from a Streptomyces species cutting at 5'-AG/GWCCT-3'.

Abstract

We isolated and characterized a new type II restriction endonuclease which recognizes the palindromic heptanucleotide sequence 5'-AGGWCCT-3' and cleaves double-stranded DNA after the first G in the sequence from a microorganism belonging to Streptomyces species. This enzyme cleaves adenovirus 2 DNA at eight sites, but does not cleave lambda phage, pBR322, pUC18 and 19, M13mp18 and 19, SV40, ColE1 and phi X174 DNAs.

Entities: Chemical Species

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Year: 1995 PMID： 7708487 PMCID： PMC306753 DOI： 10.1093/nar/23.5.742

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

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References

8 in total

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Authors: Y Ishino
Journal: Am Biotechnol Lab Date: 1992-09

2. Practical applications in molecular biology of sensitive fluorescence detection by a laser-excited fluorescence image analyzer.

Authors: Y Ishino; J Mineno; T Inoue; H Fujimiya; K Yamamoto; T Tamura; M Homma; K Tanaka; I Kato
Journal: Biotechniques Date: 1992-12 Impact factor: 1.993

3. A simple method for identifying the palindromic sequences recognized by restriction endonucleases: the nucleotide sequence of the AvaII site.

Authors: C Fuchs; E C Rosenvold; A Honigman; W Szybalski
Journal: Gene Date: 1978-09 Impact factor: 3.688

8. Purification of the glutamyl-tRNA reductase from Chlamydomonas reinhardtii involved in delta-aminolevulinic acid formation during chlorophyll biosynthesis.

Authors: M W Chen; D Jahn; G P O'Neill; D Söll
Journal: J Biol Chem Date: 1990-03-05 Impact factor: 5.157

8 in total

Sse8647I, a new type II restriction endonuclease from a Streptomyces species cutting at 5'-AG/GWCCT-3'.

1. Rapid and reliable DNA sequencing with a dideoxy sequencing kit.

2. Practical applications in molecular biology of sensitive fluorescence detection by a laser-excited fluorescence image analyzer.

3. A simple method for identifying the palindromic sequences recognized by restriction endonucleases: the nucleotide sequence of the AvaII site.

4. REBASE--restriction enzymes and methylases.

5. A general method for defining restriction enzyme cleavage and recognition sites.

6. Nucleotide sequences from the adenovirus-2 genome.

7. Cloning of the DNA polymerase gene of Bacillus caldotenax and characterization of the gene product.

8. Purification of the glutamyl-tRNA reductase from Chlamydomonas reinhardtii involved in delta-aminolevulinic acid formation during chlorophyll biosynthesis.